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ELISA Kit DIY Materials for Hyaluronic Acid (HA)

Hyaluronan; Hyaluronate

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Hyaluronic Acid (HA). No significant cross-reactivity or interference between Hyaluronic Acid (HA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Clinical Infectious Diseases Hemorrhagic Fever Caused by a Novel Bunyavirus in China: Pathogenesis and Correlates of Fatal Outcome OxfordJournals: source
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Journal of Voice Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study ScienceDirect: S0892199713002415
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European Journal of Applied Physiology The effect of aerobic walking and lower body resistance exercise on serum COMP and hyaluronan, in both males and females Pubmed:29536174
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