ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)

HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor (HGF).No significant cross-reactivity or interference between Hepatocyte Growth Factor (HGF) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Gene Therapy and Molecular Biology Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heart LWW: 01000
Journal of Translational Medicine Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma BioMed: 14795876
Digestive Diseases and Sciences Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure SpringerLink: k22823l138x2344x
Biomarker insights Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children PubMed: PMC3694825
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) PubMed: PMC3762604
BMC cell biology BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause PubMed: PMC3640998
Cell Journal (Yakhteh) Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis PubMed: PMC3584430
Gastroenterol Res Pract Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis PubMed: 26448742
Tumor Biology Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151
Cytotherapy Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury PubMed: 25863963
Biomedicine & Pharmacotherapy Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats Pubmed:27261582
Can J Physiol Pharmacol.  Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis Pubmed:27186801
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064
Changes in HGF levels after a progressive test before and after Tabata HIIT program profile:Pawel_Wolanski3
Stem Cell Research & Therapy Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats 10.1186/s13287-017-0739-3
Oncology Letters Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c‑Met/PI3K/AKT in vitro 10.3892/ol.2017.7301
Stem Cell Research & Therapy Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure. pubmed:28679425
Nature Communications Pulmonary pericytes regulate lung morphogenesis Pubmed:29934496
Experimental And Molecular Medicine Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke … Pubmed:29650950
Oncology Letters Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro Pubmed:29375714
Bulletin of Experimental Biology and Medicine Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats Pubmed:29796817
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