ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
- Product No.KSA047Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range31.2-2000pg/mL
- Applicable Sensitivity12.3pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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The Abs in the kit have high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor (HGF).No significant cross-reactivity or interference between Hepatocyte Growth Factor (HGF) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Gene Therapy and Molecular Biology||Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heart LWW: 01000|
|Journal of Translational Medicine||Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma BioMed: 14795876|
|Digestive Diseases and Sciences||Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure SpringerLink: k22823l138x2344x|
|Biomarker insights||Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children PubMed: PMC3694825|
|Biomarker insights||Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) PubMed: PMC3762604|
|BMC cell biology||BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause PubMed: PMC3640998|
|Cell Journal (Yakhteh)||Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis PubMed: PMC3584430|
|Gastroenterol Res Pract||Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis PubMed: 26448742|
|Tumor Biology||Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151|
|Cytotherapy||Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury PubMed: 25863963|
|Biomedicine & Pharmacotherapy||Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats Pubmed:27261582|
|Can J Physiol Pharmacol.||Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis Pubmed:27186801|
|Genet Mol Res||Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064|
|Changes in HGF levels after a progressive test before and after Tabata HIIT program profile:Pawel_Wolanski3|
|Stem Cell Research & Therapy||Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats 10.1186/s13287-017-0739-3|
|Oncology Letters||Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c‑Met/PI3K/AKT in vitro 10.3892/ol.2017.7301|
|Stem Cell Research & Therapy||Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure. pubmed:28679425|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|APA047Hu02||Active Hepatocyte Growth Factor (HGF)||Cell culture; Activity Assays.|
|RPA047Hu03||Recombinant Hepatocyte Growth Factor (HGF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA047Hu01||Active Hepatocyte Growth Factor (HGF)||Cell culture; Activity Assays.|
|RPA047Hu01||Recombinant Hepatocyte Growth Factor (HGF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA047Hu02||Recombinant Hepatocyte Growth Factor (HGF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA047Hu01||Polyclonal Antibody to Hepatocyte Growth Factor (HGF)||WB; IHC; ICC; IP.|
|PAA047Hu02||Polyclonal Antibody to Hepatocyte Growth Factor (HGF)||WB; IHC; ICC; IP.|
|PAA047Hu03||Polyclonal Antibody to Hepatocyte Growth Factor (HGF)||WB; IHC; ICC; IP.|
|LAA047Hu71||Biotin-Linked Polyclonal Antibody to Hepatocyte Growth Factor (HGF)||WB; IHC; ICC.|
|LAA047Hu53||APC-Linked Polyclonal Antibody to Hepatocyte Growth Factor (HGF)||WB; IHC; ICC; IF.|
|LAA047Hu81||FITC-Linked Polyclonal Antibody to Hepatocyte Growth Factor (HGF)||WB; IHC; ICC; IF.|
|MAA047Hu22||Monoclonal Antibody to Hepatocyte Growth Factor (HGF)||WB; IHC; ICC; IP.|
|SEA047Hu||ELISA Kit for Hepatocyte Growth Factor (HGF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA047Hu||CLIA Kit for Hepatocyte Growth Factor (HGF)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA047Hu01||ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)||Main materials for "Do It (ELISA Kit) Yourself".|