ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF)

Neurotrophin; Abrineurin

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF). No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Toxicological Sciences Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat Brains PubMed: 21436126
Journal of the Neurological Sciences Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord ScienceDirect: S0022510X12001219
International Conference on Bioscience Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage Psrcentre: 212059
Neuroscience Letters Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice ScienceDirect: S0304394010015788
BioMed Research International Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord. NCBI: PMC3925622
Behavioural Brain Research The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor Pubmed:25446810
Neurochemical Research Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord. Pubmed:26559686
Exp Ther Med. Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats pmc:PMC4812310
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064
The International Journal af Applied Research in Veterinary Medicine Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3
J Chem Neuroanat. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. pubmed:27914953
Int Immunopharmacol. PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. pubmed:27940378
The Journal of Maternal-Fetal & Neonatal Medicine Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease
Bipolar Disorders Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … bdi.12449
Elsevier Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls science:article
journal of allergy and clinical immunology Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitis pubmed:28412394
American Journal of Translational Research A new comorbidity model and the common pathological mechanisms of migraine and epilepsy pubmed:28559979
European Journal of Pharmacology Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal. pubmed:29042206
Brain, Behavior, and Immunity An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling Pubmed:29627530
Stem Cell Research & Therapy Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model Pubmed:29335016
Pharmacological Research  N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype Pubmed:29203442
Experimental Neurology Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia Pubmed:30012511
Nutrients Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model. Pubmed:29890692
Neurotoxicity Research Delta-9-Tetrahydrocannabinol (∆-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley Rats Pubmed:28933048
Neurotoxicology Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats Pubmed:29522778
Neuroscience Letters Long term Westernized diet leads to region-specific changes in brain signaling mechanisms Pubmed:29655945
Catalog No. Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse) Organism species Applications (RESEARCH USE ONLY!)
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