ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF)
- Product No.KSA011Mi01
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range31.2-2000pg/mL
- Applicable Sensitivity12.6pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- UOM 96T*596T*10 96T*20 96T*50 96T*100
For more details, please contact local distributors! US$ 1869
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The Abs in the kit have high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF). No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Toxicological Sciences||Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat Brains PubMed: 21436126|
|Journal of the Neurological Sciences||Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord ScienceDirect: S0022510X12001219|
|International Conference on Bioscience||Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage Psrcentre: 212059|
|Neuroscience Letters||Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice ScienceDirect: S0304394010015788|
|BioMed Research International||Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord. NCBI: PMC3925622|
|Behavioural Brain Research||The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor Pubmed:25446810|
|Neurochemical Research||Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord. Pubmed:26559686|
|Exp Ther Med.||Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats pmc:PMC4812310|
|Genet Mol Res||Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064|
|The International Journal af Applied Research in Veterinary Medicine||Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3|
|J Chem Neuroanat.||Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. pubmed:27914953|
|Int Immunopharmacol.||PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. pubmed:27940378|
|The Journal of Maternal-Fetal & Neonatal Medicine||Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639|
|IOS Press Content Library||An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease|
|Bipolar Disorders||Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … bdi.12449|
|Elsevier||Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls science:article|
|journal of allergy and clinical immunology||Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitis pubmed:28412394|
|American Journal of Translational Research||A new comorbidity model and the common pathological mechanisms of migraine and epilepsy pubmed:28559979|
|European Journal of Pharmacology||Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal. pubmed:29042206|
|Brain, Behavior, and Immunity||An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling Pubmed:29627530|
|Stem Cell Research & Therapy||Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model Pubmed:29335016|
|Pharmacological Research||N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype Pubmed:29203442|
|Experimental Neurology||Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia Pubmed:30012511|
|Nutrients||Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model. Pubmed:29890692|
|Neurotoxicity Research||Delta-9-Tetrahydrocannabinol (∆-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley Rats Pubmed:28933048|
|Neurotoxicology||Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats Pubmed:29522778|
|Neuroscience Letters||Long term Westernized diet leads to region-specific changes in brain signaling mechanisms Pubmed:29655945|
|Catalog No.||Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA011Mi01||Recombinant Brain Derived Neurotrophic Factor (BDNF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA011Mi01||Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)||WB; IHC; ICC; IP.|
|MAA011Mi21||Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)||WB; IHC; ICC; IP.|
|MAA011Mi26||Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)||WB; IHC; ICC; IP.|
|LAA011Mi76||Biotin-Linked Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)||WB; IHC; ICC.|
|SEA011Mi||ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PSA011Mi01||Antibody Pair for Brain Derived Neurotrophic Factor (BDNF)||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSA011Mi01||ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF)||Main materials for "Do It (ELISA Kit) Yourself".|