Instant ELISA Kit for Estradiol (E2)
17B-Estradiol; Oestradiol; Beta-Estradiol
- Product No.IEA461Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1h, 10min
- Detection Range12.35-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.65pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 507
For more details, please contact local distributors! US$ 2282
For more details, please contact local distributors! US$ 4310
For more details, please contact local distributors! US$ 35490
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Instant Estradiol (E2).
No significant cross-reactivity or interference between Instant Estradiol (E2) and analogues was observed.
Matrices listed below were spiked with certain level of Instant Estradiol (E2) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Estradiol (E2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Estradiol (E2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Estradiol (E2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Estradiol (E2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
|BioMed research international||Antiosteoclastic Activity of Milk Thistle Extract after Ovariectomy to Suppress Estrogen Deficiency-Induced Osteoporosis PubMed: PMC3678416|
|Fertility and Sterility||Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356|
|Repositório Digital||Utilizacao de células-tronco adultas para tratamento de falência ovariana causada por quimioterapia em camundongas Lume: Source|
|Electromagnetic Biology and Medicine||The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source|
|Academic Sciences||Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Ijppsjournal: Source|
|Stem Cells Int.||Cell Therapy for Chemically Induced Ovarian Failure in Mice Pubmed:Pmc4274854|
|Reproductive Toxicology||Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring Pubmed:24126190|
|Phytomedicine.||Phloretin promotes osteoclast apoptosis in murine macrophages and inhibits estrogen deficiency-induced osteoporosis in mice Pubmed:24932975|
|Turkish Journal of Urology||Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source|
|international journal of environmental research and public health||Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy PubMed: 25996886|
|Reprod Toxicol||In utero PubMed: 25530038|
|oncology research||Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940|
|Environ Toxicol||Effect on the H19 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period PubMed: 25782373|
|J Endocrinol||Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction PubMed: 25770118|
|Reproduction||Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in rats PubMed: 26556892|
|Veterinary Word||Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212|
|Archives of Physiology and Biochemistry||Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364|
|Plos One||Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota Pubmed:27093642|
|Biology of Reproduction||Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114|
|PPAR Research||Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα Pubmed:27413362|
|Clinical and Experimental Hypertension||Assessment of ADMA, estradiol, and progesterone in severe preeclampsia Pubmed:27152507|
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|Scientific Reports||The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization pubmed:27876896|
|Scientific Reports||Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373|
|33||Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389|
|African Journal of Environmental Science and Technology||Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 150659|
|Journal of Functional Foods||Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561|
|environmental pollution||Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061|
|Environmental toxicology and pharmacology||Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats. pubmed:28376377|
|PPAR Research||Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362|
|Toxicological Sciences||Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384|
|Sci Rep||Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267|
|Interact Cardiovasc Thorac Surg||Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats pubmed:29049608|
|Toxicological Sciences||Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction pubmed:28973666|
|Reproductive Sciences||Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome pubmed:28673204|
|Oncotarget||Rcan2 and estradiol independently regulate body weight in female mice pubmed:28624805|
|African Journal of Environmental Science and Technology||Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 10.5897/AJEST2016.2234|
|Journal of Orthopaedic Research||The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice pubmed:29058779|
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|Asian Journal of Biomedical and Pharmaceutical Sciences||A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/ÃÂ²-catenin Signaling Pathways in Ovariectomized Rats ISSN 2249 – 622X|
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|Molecular Medicine Reports||Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells pubmed:28656272|
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