Instant ELISA Kit for Estradiol (E2)

17B-Estradiol; Oestradiol; Beta-Estradiol

Specificity

This assay has high sensitivity and excellent specificity for detection of Instant Estradiol (E2).
No significant cross-reactivity or interference between Instant Estradiol (E2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Instant Estradiol (E2) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Estradiol (E2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-96 91
EDTA plasma(n=5) 92-105 95
heparin plasma(n=5) 93-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Estradiol (E2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Estradiol (E2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Estradiol (E2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 88-96% 98-105% 78-97%
EDTA plasma(n=5) 94-104% 78-98% 79-94% 82-95%
heparin plasma(n=5) 80-92% 84-91% 82-99% 80-91%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.

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Biology of Reproduction Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114
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Clinical and Experimental Hypertension Assessment of ADMA, estradiol, and progesterone in severe preeclampsia Pubmed:27152507
PLoS Genet. A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis Pubmed:26977770
Scientific Reports The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization pubmed:27876896
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389
African Journal of Environmental Science and Technology Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 150659
Journal of Functional Foods Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061
Environmental toxicology and pharmacology Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats. pubmed:28376377
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362
Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384
Sci Rep Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267
Interact Cardiovasc Thorac Surg Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats pubmed:29049608
Toxicological Sciences Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction pubmed:28973666
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Oncotarget Rcan2 and estradiol independently regulate body weight in female mice pubmed:28624805
African Journal of Environmental Science and Technology Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 10.5897/AJEST2016.2234
Journal of Orthopaedic Research The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice pubmed:29058779
Animal Production Science Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? 10.1071/ANv57n12Ab065
Asian Journal of Biomedical and Pharmaceutical Sciences A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/β-catenin Signaling Pathways in Ovariectomized Rats ISSN 2249 – 622X
Nanotheranostics The biocompatibility studies of polymer dots on pregnant mice and fetuses pubmed:29071192
DARU Journal of Pharmaceutical Sciences Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999
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Molecular Medicine Reports Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells pubmed:28656272
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