Instant ELISA Kit for Interferon Alpha (IFNa)

IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D

Specificity

This assay has high sensitivity and excellent specificity for detection of Instant Interferon Alpha (IFNa).
No significant cross-reactivity or interference between Instant Interferon Alpha (IFNa) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Instant Interferon Alpha (IFNa) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Interferon Alpha (IFNa) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-102 87
EDTA plasma(n=5) 88-101 93
heparin plasma(n=5) 96-103 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Interferon Alpha (IFNa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Interferon Alpha (IFNa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-95% 78-88% 94-102% 92-101%
EDTA plasma(n=5) 89-103% 80-92% 83-97% 98-105%
heparin plasma(n=5) 85-105% 78-104% 92-101% 82-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 5 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Animal and Veterinary Advances Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine Medwell: 2010610616
Journal of Animal and Veterinary Advances Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus Medwell: 2010594600
Ann Rheum Dis. The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats PubMed: 23172753
Nanomedicine Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform PubMed: 23394132
International journal of experimental pathology Experimental rat model for alcohol-induced osteonecrosis of the femoral head PubMed: PMC3781777
Antiviral Research A recombinant adenovirus bicistronically expressing porcine interferon-α and interferon-γ enhances antiviral effects against foot-and-mouth disease virus ScienceDirect: S0166354214000266
British Journal of Pharmacology Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells Onlinelibrary: bph.12440
BMC Cancer High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model Pubmed:24950740
PLoS One. Respiratory Mechanics and Plasma Levels of Tumor Necrosis Factor Alpha and Interleukin 6 Are Affected by Gas Humidification during Mechanical Ventilation in Dogs Pubmed:25036811
Veterinary Research Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain Pubmed:25106750
Asian Pac J Trop Med Effect of SecinH3 on lung injury induced by sepsis of rats PubMed: 26706678
PLoS One siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro Pubmed:26886455
Plos One Sulforaphane Suppresses Hepatitis C Virus Replication by Up-ReCavia (Guinea pig )lating Heme Oxygenase-1 Expressionthrough PI3K/Nrf2 Pathway. Pubmed:27023634
PLoS One. Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in Cavia (Guinea pig )inea pigs. Pubmed:23776655
Veterinary microbiology Impact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain). pubmed:27599928
International Journal of Clinical and Experimental Pathology Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells files:ijcep0036479.pdf
Scandinavian Journal of Immunology Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress. pubmed:27384769
Saudi Journal of Gastroenterology Deoxyschizandrin suppresses dss-induced ulcerative colitis in mice. pubmed:27976641
Biochemical and Biophysical Research Communications MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B pubmed:28342861
Virology The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection pubmed:28988058
Antiviral Research Coinjection of a vaccine and anti-viral agents can provide fast-acting protection from foot-and-mouth disease pubmed:28454913
Molecular Therapy. Methods & Clinical Development Anti-high mobility group box 1 antibody ameliorates albuminuria in MRL/lprlupus-prone mice pubmed:28649578
Journal of Virology Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation. pubmed:28915824
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA033Ca02 Recombinant Interferon Alpha (IFNa) Positive Control; Immunogen; SDS-PAGE; WB.
RPA033Ca01 Recombinant Interferon Alpha (IFNa) Positive Control; Immunogen; SDS-PAGE; WB.
PAA033Ca02 Polyclonal Antibody to Interferon Alpha (IFNa) WB; IHC; ICC; IP.
PAA033Ca01 Polyclonal Antibody to Interferon Alpha (IFNa) WB; IHC; ICC; IP.
LAA033Ca81 FITC-Linked Polyclonal Antibody to Interferon Alpha (IFNa) WB; IHC; ICC; IF.
LAA033Ca71 Biotin-Linked Polyclonal Antibody to Interferon Alpha (IFNa) WB; IHC; ICC.
SEA033Ca ELISA Kit for Interferon Alpha (IFNa) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA033Ca Instant ELISA Kit for Interferon Alpha (IFNa) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA033Ca CLIA Kit for Interferon Alpha (IFNa) Chemiluminescent immunoassay for Antigen Detection.