High Sensitive ELISA Kit for Toll Like Receptor 9 (TLR9)
- Product No.HEA709Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 12.1pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of High Sensitive Toll Like Receptor 9 (TLR9).
No significant cross-reactivity or interference between High Sensitive Toll Like Receptor 9 (TLR9) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive Toll Like Receptor 9 (TLR9) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Toll Like Receptor 9 (TLR9) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Toll Like Receptor 9 (TLR9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Toll Like Receptor 9 (TLR9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Toll Like Receptor 9 (TLR9) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Chinese Medical Journal||Expression of soluble Toll-like receptors in pleural effusions CMJ: 2010823483999506297|
|DNA Cell Biol.||Expression of serum toll-like receptor 9 and oxidative damage markers in benign and malignant breast diseases Pubmed:24906089|
|Journal of Trace Elements in Medicine and Biology||Correlation of serum toll like receptor 9 and trace elements with lipid peroxidation in the patients of breast diseases PubMed: 25744504|
|Journal of cancer research and clinical oncology||TLR9 gene polymorphism− 1486T/C (rs187084) is associated with uterine cervical neoplasm in Mexican female population pubmed:28819773|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
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|HEA709Hu||High Sensitive ELISA Kit for Toll Like Receptor 9 (TLR9)||Enzyme-linked immunosorbent assay for Antigen Detection.|
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|SCA709Hu||CLIA Kit for Toll Like Receptor 9 (TLR9)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA709Hu01||ELISA Kit DIY Materials for Toll Like Receptor 9 (TLR9)||Main materials for "Do It (ELISA Kit) Yourself".|