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Rat Model for Cerebral Thrombosis (CT)

CVST; Cerebral venous sinus thrombosis

  • Product No.DSI683Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Prototype SpeciesHuman
  • SourceAutologous blood emboli
  • Model Animal StrainsWistar Rats(SPF), healthy, male and female, body weight 280g~300g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period4-6 weeks
  • Modeling Method"Rat, weight280 ~ 300g, males and females. Prepare a PE-50 catheter through a hose then micro-sampler, filled with thrombin before surgery. Intraperitoneal injection of pentobarbital sodium (by 50 ~ 60 mg / Kg body weight dose) or chloral hydrate (according to the dose of 350 ~ 400 mg / kg body weight) after anesthesia, the animal supine fixed on the operating table, shaving neck hair, surgical area disinfection. (CCA), left external carotid artery (ECA). Isolated from the left side of the left ECA branch, for the carotid artery bifurcation distal 7 ~ 10 mm department of the left common carotid artery The ECA was ligated and dislocated with a 5- 0 nylon thread, and the distal thread was pulled down to align it with the internal carotid artery (ICA). Separate the left side of the 1CA, gently stripping the vagus nerve, Unique extracranial branching of the palatal artery, ligation of the artery, so that ICA CCA extracorporeal only to retain the artery with micro-arterial clip clip ICA and CCA, with nylon line in the ECA start to play a loose, in the ECA on the neck The arterial bifurcation is cut at about 3 mm from the mouth, and the catheter is inserted into the ECA lumen and the ICA is inserted into the ICA to tighten the nylon thread at the beginning of the ECA to prevent movement and bleeding of the catheter. Remove the microcavity clamp on the ICA Insert the catheter about 10mm, at this time, the catheter from the beginning of the MCA from the beginning of 2 ~ 3 mm. Extract about 10ul of arterial blood into the catheter, stay about 10 min to form an embolus, temporarily with a micro-arterial clip occlusion of the contralateral CCA to reduce the brain Blood flow, then the catheter in the emboli with about 10 U thrombin slowly injected into the ICA, 5 min after removal of the right side of the CCA on the micro-arterial clip; after 10 min, pull out the catheter, ligation on the ECA proximal; 15 min later remove the left side of the CCA on the micro-arterial clip, suture the skin."
  • Applicationsn/a
  • Downloadn/a
  • UOM Each case
  • FOB US$ 214 
    For more details, please contact local distributors!

Model Evaluation

Pathological Results

Cytokines Level

Statistical Analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
DSI683Ra01 Rat Model for Cerebral Thrombosis (CT) n/a
DSI683Ra02 Rat Model for Cerebral Thrombosis (CT) n/a