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Rat Model for Alcoholic Hepatitis (AH)

Alcoholic Liver Disease

  • Product No.DSI531Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Prototype SpeciesHuman
  • SourceChronic alcoholic liver disease (ALD) induced by long term intake of alcohol
  • Model Animal StrainsSD Rats (SPF), healthy, male, bodyweight:180g~200g
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group (three doses).
  • Modeling Period12 w
  • Modeling Method1. Rats in normal group were free to drink water.
    2. Rats in alcoholic fatty liver disease(AFL) group drink alcohol, the alcohol concentration from the beginning of 5%, each concentration was maintained for a week, in order to 10%, 15%, 20%, 25%, 30%, 35% to 40%, with 40% concentration lasted for 12 weeks.
    3. On the course of the experiment, the rats in each group were given ordinary pellet feed for 12 weeks.
    4. At the end of the 12th week, the animals were fasted for 12 hours, weighed, and take the blood from the inferior vena cava.
  • ApplicationsUsed to study the pathogenesis of alcoholic liver disease and to screen the drugs for prevention and treatment of alcoholic liver disease
  • Downloadn/a
  • UOM Each case
  • FOB US$ 240 
    For more details, please contact local distributors!

Model Evaluation

1. Detection of blood biochemical parameters
The contents of ALT, AST, TC, TG, FFA, LDL-C, HDL-C and blood glucose.

Pathological Results

2. Histopathological changes of liver
The results showed that the volume of liver in rats with alcoholic fatty liver and sucrose was significantly increased
Swelling, membrane tension, the edge of the round blunt, milk yellow, greasy section, especially in the abdominal cavity after the accumulation of retroperitoneal fat
Obvious.
HE staining showed that there was no obvious fatty degeneration in the liver of the normal group, and the hepatic lobule structure was normal. In the AFL model group, the liver was filled with a lot of fat degeneration, and the volume of the cells increased.

Cytokines Level

The content of TNF-α and ApoB in serum were detected by ELISA method.

Statistical Analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
DSI531Ra02 Rat Model for Alcoholic Hepatitis (AH) n/a
DSI531Ra01 Rat Model for Alcoholic Hepatitis (AH) Used to study the pathogenesis of alcoholic liver disease and to screen the drugs for prevention and treatment of alcoholic liver disease