Packages (Simulation)
Reagent Preparation
Image (I)
-
Results demonstration
Image (II)
-
Typical Standard Curve
Quality Guarantee
Instruction Manual
Certificate
Maybe you can also pay attention to our smart microplate reader
ELISA Kit for Beta-Hydroxybutyric Acid (bHB)
OHB; BHBA; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid; β-Hydroxybutyric acid
- Product No.CEB022Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range1.23-100ug/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.44ug/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 565
US$ 808
US$ 3634
US$ 6864
US$ 56525
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Beta-Hydroxybutyric Acid (bHB).
No significant cross-reactivity or interference between Beta-Hydroxybutyric Acid (bHB) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Beta-Hydroxybutyric Acid (bHB) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-Hydroxybutyric Acid (bHB) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-97 | 90 |
| EDTA plasma(n=5) | 89-105 | 98 |
| heparin plasma(n=5) | 81-90 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Hydroxybutyric Acid (bHB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Hydroxybutyric Acid (bHB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-Hydroxybutyric Acid (bHB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-97% | 97-105% | 97-105% | 96-104% |
| EDTA plasma(n=5) | 84-92% | 95-103% | 95-103% | 86-101% |
| heparin plasma(n=5) | 85-102% | 84-103% | 79-89% | 95-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
GIVEAWAYS
INCREMENT SERVICES
| Magazine | Citations |
| Endocrinology | Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression Endocrine:Source |
| Chem Commun (Camb) | A bioelectronic system for insulin release triggered by ketone body mimicking diabetic ketoacidosis in vitro PubMed: 25846235 |
| PLoS One | Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine pubmed:27501393 |
| Buffalo Bulletin | Alterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes |
| Journal of Integrative Agriculture | Temporal dynamics of nutrient balance, plasma biochemical and immune traits, and liver function in transition dairy cows |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CPB022Ge11 | BSA Conjugated Beta-Hydroxybutyric Acid (bHB) | Immunogen; SDS-PAGE; WB. |
| CPB022Ge21 | OVA Conjugated Beta-Hydroxybutyric Acid (bHB) | Immunogen; SDS-PAGE; WB. |
| PAB022Ge01 | Polyclonal Antibody to Beta-Hydroxybutyric Acid (bHB) | WB; IHC; ICC; IP. |
| CEB022Ge | ELISA Kit for Beta-Hydroxybutyric Acid (bHB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB022Ge | Multiplex Assay Kit for Beta-Hydroxybutyric Acid (bHB) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSB022Ge11 | ELISA Kit DIY Materials for Beta-Hydroxybutyric Acid (bHB) | Main materials for "Do It (ELISA Kit) Yourself". |