ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
- Product No.CEA551Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.88pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 608
For more details, please contact local distributors! US$ 2736
For more details, please contact local distributors! US$ 5168
For more details, please contact local distributors! US$ 42560
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2).
No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 2, Basic (FGF2) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 2, Basic (FGF2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 2, Basic (FGF2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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|International Journal of Nanomedicine||Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis PubMed: PMC3254262|
|Archives of Animal Nutrition||Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesis Tandfonline: 644918|
|Diabetes||Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb Ischemia Pubmed: 22266669|
|Molecular Pharmacology||Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic Rats Pubmed: 22091745|
|Science Alert||Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats Scialert: Source|
|Nanoscale Research Letters||Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart. PubMed: 22827927|
|PLoS One||Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis PubMed: PMC3771850|
|International Journal of Molecular Sciences||Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles NCBI:PMC3856104|
|Archives of Animal Nutrition||Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesis Pubmed: 23952606|
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|PLoS One.?||Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate Neurotoxicity Pubmed:25426952|
|Implant Dentistry||Effects of Hemostatic Agents on Fibroblast Cells Lww:Source|
|Journal of Materials Chemistry B||bFGF-grafted electrospun fibrous scaffolds via poly(dopamine) for skin wound healing Rsc:Source|
|Archives of Animal Nutrition||Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos Pubmed:25530495|
|Int J Clin Exp Med.||The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972|
|Theriogenology||Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes Science: Article|
|Tumor Biology||Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151|
|Acta Biomaterialia||Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosa article:S1742706115301410|
|22||Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars Pubmed:26774564|
|Genet Mol Res||Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064|
|BMC Neuroscience||3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPA pubmed:27456338|
|Medical Science Monitor||5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts 898221|
|Biomedicine & Pharmacotherapy||FGF-2-mediated FGFR1 signaling in human microvascular endothelial cells is activated by vaccarin to promote angiogenesis pubmed:28841454|
|Medical Science Monitor||5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts pubmed:28052053|
|International Journal of Clinical and Experimental Pathology||Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424|
|Journal of Biomaterials and Tissue Engineering||The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice 10.1166/jbt.2017.1652|
|Stem Cells and Development||Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular Restoration Pubmed:29630447|
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