ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

NT-Pro-BNP; ; N-BNP

Specificity

This assay has high sensitivity and excellent specificity for detection of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP).
No significant cross-reactivity or interference between N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and the recovery rates were calculated by comparing the measured value to the expected amount of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-101 95
EDTA plasma(n=5) 78-102 89
heparin plasma(n=5) 80-93 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-93% 81-89% 90-102% 80-104%
EDTA plasma(n=5) 80-91% 85-92% 97-105% 90-97%
heparin plasma(n=5) 98-105% 92-101% 80-95% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Eur Rev Med Pharmacol Sci. The role of serum n-terminal pro-brain natriuretic peptide in transient tachypnea of the newborn Europeanreview: Source
Transplantation Proceedings Vascular Adhesion Protein-1, a Novel Molecule, in Kidney and Heart Allograft Recipients Pubmed: 23769096
34 Paradoxical low-flow, low-gradient aortic stenosis despite preserved left ventricular ejection fraction: new insights from weights of operatively excised aortic valves Pubmed: 24755006
Integrative Medicine Insights A Randomized Controlled Trial to Study the Effect of Yoga Therapy on Cardiac Function and N Terminal Pro BNP in Heart Failure NCBI: PMC3981569
Liver Int.? Diabetes enhances the intrahepatic vascular response to endothelin-1 in cirrhotic rats: association with the ETA receptor and pERK up-regulation. Pubmed:24636620
Eur J Heart Fail. Growth-differentiation factor 15 and osteoprotegerin in acute myocardial infarction complicated by cardiogenic shock: a biomarker substudy of the IABP-SHOCK II-trial. Pubmed:24903195
Scientific Reports Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153
Int J Mol Sci The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599
scientific reports Effect of Treatment on Body Fluid in Patients with Unilateral Aldosterone Producing Adenoma: Adrenalectomy versus Spironolactone PubMed: 26477337
Liver Int Diabetes enhances the intrahepatic vascular response to endothelin‐1 in cirrhotic rats: association with the ETA receptor and pERK up‐regulation PubMed: 24636620
Int J Clin Exp Pathol Qiliqiangxin improves cardiac function and attenuates cardiac remodeling in rats with experimental myocardial infarction PubMed: 26261541
Iranian Journal of Pediatrics NT-proBNP in Children With Left to Right Shunt and Dilated Cardiomyopathy serve:4485
International Journal of Cardiology Human myoblast transplantation in mice infarcted heart alters the expression profile of cardiac genes associated with left ventricle remodeling Pubmed:26457413
IJC Heart & Vasculature Cardiovascular changes in patients with non-severe Plasmodium vivax malaria article:S2352-9067(16)30010-0
Heart Lung Circ Soluble Glycoprotein 130 and Heat Shock Protein 27 as Novel Candidate Biomarkers of Chronic Heart Failure with Preserved Ejection Fraction Pubmed:27067668
International journal of cardiology Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies. pubmed:27448535
Experimental and Therapeutic Medicine Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure. pubmed:28565777
Journal of Cardiovascular Electrophysiology Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. pubmed:28127817
Journal of Cardiovascular Ecography Study of Vitamin D Status in Patients with Dilated Cardiomyopathy at a Teaching Hospital in North India. pubmed:28465969
International Journal of Cardiology 5-methoxytryptophan is a potential marker for post-myocardial infarction heart failure - apreliminary approach to clinical utility. pubmed:27526355
Cardiovasc Toxicol Beneficial Effects of Ozone Therapy on Oxidative Stress, Cardiac Functions and Clinical Findings in Patients with Heart Failure Reduced Ejection Fraction pubmed:28097518
Sci Rep.  Serial heart rhythm complexity changes in patients with anterior wall ST segment elevation myocardial infarction. pubmed:28252107
Journal of Cardiovascular Magnetic Resonance The predictive capabilities of a novel cardiovascular magnetic resonance derived marker of cardiopulmonary reserve on established prognostic surrogate markers in patients with pulmonary vascular disease: results of a longitudinal pilot study pubmed:28065166
Repositório Institucional UNESP AVALIAÇÃO ELETROCARDIOGRÁFICA AMBULATORIAL DE CÃES COM EHRLIQUIOSE MONOCÍTICA CRÔNICA handle:11449
Archivos de medicina veterinaria Cardiac biomarkers in dogs with visceral leishmaniasis S0301-732X2016000300004
Original article Evaluation of N-Terminal Pro Brain Natriuretic Peptide as a biomarker for clinical severity of heart failure in pediatric population Attach432
Evidence-Based Complementary and Alternative Medicine Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway pubmed:28698735
Scientific Reports QSKL protects against myocardial apoptosis on heart failure via PI3K/Akt-p53 signaling pathway. pubmed:29209026
Vascular Pharmacology Reversal effects of low-dose imatinib compared with sunitinib on monocrotaline-induced pulmonary and right ventricular remodeling in rats pubmed:29100963
Evidence-based complementary and alternative medicine Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Rats pubmed:28706558
Europace Prediction of electro-anatomical substrate using APPLE score and biomarkers Pubmed:29893827
Heart Rhythm Role of NT-proANP and NT-proBNP in patients with atrial fibrillation: Association with atrial fibrillation progression phenotypes Pubmed:29604419
American Journal of Hypertension MicroRNAs in Peripheral Mononuclear Cells as Potential Biomarkers in Hypertensive Patients With Heart Failure With Preserved Ejection Fraction Pubmed:29506053
International Journal of Molecular Medicine Differential microRNA expression profiles and bioinformatics analysis between young and aging spontaneously hypertensive rats Pubmed:29328372
Biomarkers Regulation of circulating chromogranin B levels in heart failure Pubmed:29098879
Lung Effect of 6-min Walk Test on pro-BNP Levels in Patients with Pulmonary Arterial Hypertension Pubmed:29564533
International Journal of Clinical and Exp Medicine Dynamic monitoring the level of NT-proBNP in assessing the value of cardiac function and prognosis in patients with systemic infection ijcem0072283.pdf
FASEB Journal Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922
International Journal of Clinical and Experimental Medicine Qiliqiangxin ameliorates cardiac dysfunction in heart failure rats post myocardial infarction via activation of HIF/VEGF pathway
Pediatric Hematology Oncology Journal Decreasing cardiac iron overload with Amlodipine and Spirulina in children with β-thalassemia Doi: 10.1016/j.phoj.2018.10.001
Journal of Clinical Medicine Acute Responses of Novel Cardiac Biomarkers to a 24-h Ultra-Marathon Pubmed: 30625976
Van Veterinary Journal Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure.
Journal of Complementary and Integrative Medicine Biological markers for the effects of yoga as a complementary and alternative medicine Pubmed: 30735481
Frontiers in Pharmacology Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway Pubmed: 30890937
Journal of Clinical Medicine Potential Effects of CXCL9 and CCL20 on Cardiac Fibrosis in Patients with Myocardial Infarction and Isoproterenol-Treated Rats Pubmed: 31083544
PLoS One Evaluation of heart rate variability and behavior of electrocardiographic parameters in dogs affected by chronic Monocytic Ehrlichiosis Pubmed: 31125348
Cardiovascular Research Cardio-protective effect of the secretome of Sca-1+ and Sca-1-cells in heart failure–not equal, but equally important? Paracrine cell effects on heart failure
Advances in Medical Sciences The potential role of inflammation in cryptogenic stroke
Disease Markers Arterial Stiffness and Indices of Left Ventricular Diastolic Dysfunction in Patients with Embolic Stroke of Undetermined Etiology Pubmed: 31612072
Journal of Human Kinetics Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy Pubmed: 32148580
Nature Communications Preclinical development of a miR-132 inhibitor for heart failure treatment Pubmed: 32005803
MEDIATORS OF INFLAMMATION Evaluating the Relationship of GDF-15 with Clinical Characteristics, Cardinal Features, and Survival in Multiple Myeloma Pubmed: 33144847
EUROPEAN HEART JOURNAL CDR132L improves systolic and diastolic function in a large animal model of chronic heart failure Pubmed: 33089304
REPRODUCTIVE TOXICOLOGY Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult mice Pubmed: 33068716
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Apela improves cardiac and renal function in mice with acute myocardial infarction Pubmed: 32686917
J Multidiscip Healthc Left Ventricular Diastolic Dysfunction as Predictor of Unfavorable Prognosis After ESUS 33727824
Oxid Med Cell Longev κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a 34373768
Biology Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy 34356497
Arch Acad Emerg Med Risk Stratification of Pulmonary Thromboembolism using Brain Natriuretic Peptide and Troponin I; a Brief Report Pubmed:35072097
Transl Res Interplay between mineral bone disorder and cardiac damage in acute kidney injury: from Ca2+ mishandling and preventive role of Klotho in mice to its potential … Pubmed:35077866
Medicina (Kaunas) The Key Role of Hepcidin-25 in Anemia in Multiple Myeloma Patients with Renal Impairment Pubmed:35334593
Acta Cardiol Sin Prothymosin α Gene Transfer Modulates Myocardial Remodeling after Ischemia-Reperfusion Injury Pubmed:35273440
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APA485Mu61 Active N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Cell culture; Activity Assays.
EPA485Mu62 Eukaryotic N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Positive Control; Immunogen; SDS-PAGE; WB.
RPA485Mu01 Recombinant N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Positive Control; Immunogen; SDS-PAGE; WB.
CPA485Mu21 OVA Conjugated N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Immunogen; SDS-PAGE; WB.
CPA485Mu31 KLH Conjugated N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Immunogen; SDS-PAGE; WB.
PAA485Mu08 Polyclonal Antibody to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) WB; IHC; ICC; IP.
SEA485Mu ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA485Mu ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA485Mu Mini Samples ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA485Mu Multiplex Assay Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.