ELISA Kit for Thyroid Stimulating Hormone (TSH)
- Product No.CEA463Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range185.2-15,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 72.4pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 720
For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors! US$ 6120
For more details, please contact local distributors! US$ 50400
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone (TSH).
No significant cross-reactivity or interference between Thyroid Stimulating Hormone (TSH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Thyroid Stimulating Hormone (TSH) and the recovery rates were calculated by comparing the measured value to the expected amount of Thyroid Stimulating Hormone (TSH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thyroid Stimulating Hormone (TSH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Blood||Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID Pubmed: 22184407|
|Life Science Journal||Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies Lifesciencesite: Source|
|Thyroid||Impact of Metformin and Compound C on NIS expression and iodine uptake in vitro and in vivo: a role for CRE in AMPK modulation of thyroid function Pubmed: 23819433|
|Journal of Functional Foods||Effect of lettuce biofortified with iodine by soil fertilization on iodine concentration in various tissues and selected biochemical parameters in serum of Wistar rats Sciencedirect:S1756464615000894|
|J Endocrinol.||Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis Pubmed:24928937|
|journal of lipid research||Thyroid-stimulating hormone decreases HMG-CoA reductase phosphorylation via AMP-activated protein kinase in the liver PubMed: 25713102|
|Int J Clin Exp Med||Lead exposure causes thyroid abnormalities in diabetic rats PubMed: 26221254|
|Jubilant Drax Image||Perinatal Iron Deficiency-Induced Hypothyroxinemia Impairs Early Brain Development Regardless of Normal Iron Levels in the Neonatal Brain Pubmed:27231981|
|PLoS One.||The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats Pubmed:27043135|
|journal of bone and mineral research||Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis. pubmed:27589370|
|Toxicological Sciences||Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384|
|Sci Rep||Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267|
|Bulletin of the World Health Organization||Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders handle:123456789|
|International Journal of Peptide Research and Therapeutics||Effect of Intracerebroventricular Administration of Apelin-13 on the Hypothalamus–Pituitary–Thyroid Axis and Peripheral Uncoupling Proteins article:10.1007|
|Tropical Journal of Pharmaceutical Research||Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats 10.4314/tjpr.v16i5.9|
|30||The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. pubmed:28546511|
|Catalog No.||Related products for research use of Canis familiaris; Canine (Dog) Organism species||Applications (RESEARCH USE ONLY!)|
|CEA463Ca||ELISA Kit for Thyroid Stimulating Hormone (TSH)||Enzyme-linked immunosorbent assay for Antigen Detection.|