ELISA Kit for Alpha-Melanocyte Stimulating Hormone (aMSH)
α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin
- Product No.CEA239Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range123.5-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 44.1pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Alpha-Melanocyte Stimulating Hormone (aMSH).
No significant cross-reactivity or interference between Alpha-Melanocyte Stimulating Hormone (aMSH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Alpha-Melanocyte Stimulating Hormone (aMSH) and the recovery rates were calculated by comparing the measured value to the expected amount of Alpha-Melanocyte Stimulating Hormone (aMSH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Melanocyte Stimulating Hormone (aMSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Melanocyte Stimulating Hormone (aMSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|J Biochem.||Generation of a human bone marrow-derived mesenchymal stem cell line expressing and secreting high levels of bioactive α-melanocyte-stimulating hormone. Pubmed: 23341471|
|Pigment Cell Melanoma Res||Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit regulated by light PubMed: 26582755|
|The Journal of Biological Chemistry||Intermedin Restores Hyperhomocysteinemia-induced Macrophage Polarization and Improves Insulin Resistance in Mice Pubmed:27080257|
|Pigment Cell & Melanoma Research||Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit reCavia (Guinea pig )lated by light Pubmed:26582755|
|Nature Communications||Endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human pluripotent stem cell derivatives. pubmed:28794470|
|Cell Stem Cell||Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses Pubmed:29681516|
|European Neuropsychopharmacology||Epigenetic alterations of the POMC promoter in tobacco dependence Pubmed:29871818|
|Neuropeptides||Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor Pubmed:29685637|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|CEA239Mu||ELISA Kit for Alpha-Melanocyte Stimulating Hormone (aMSH)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA239Mu11||ELISA Kit DIY Materials for Alpha-Melanocyte Stimulating Hormone (aMSH)||Main materials for "Do It (ELISA Kit) Yourself".|