ELISA Kit for Free Thyroxine (fT4)

FTI

Specificity

This assay has high sensitivity and excellent specificity for detection of Free Thyroxine (fT4).
No significant cross-reactivity or interference between Free Thyroxine (fT4) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Free Thyroxine (fT4) and the recovery rates were calculated by comparing the measured value to the expected amount of Free Thyroxine (fT4) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-93 88
EDTA plasma(n=5) 80-89 82
heparin plasma(n=5) 78-103 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Free Thyroxine (fT4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Free Thyroxine (fT4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Free Thyroxine (fT4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-97% 78-93% 79-104% 86-101%
EDTA plasma(n=5) 90-99% 90-104% 91-101% 94-105%
heparin plasma(n=5) 95-102% 78-89% 88-95% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Frontiers in cellular neuroscience Elevated Thyroid Peroxidase Antibody Increases Risk of Post-partum Depression by Decreasing Prefrontal Cortex BDNF and 5-HT Levels in Mice pubmed:28119573
ARC Journal of Nutrition and Growth Effect of Selenium and Zinc Supplementation on Hypothyroidism in Rats ISSN 2455-2550
Frontiers in Neuroscience Modulation of Calcitonin, Parathyroid Hormone, and Thyroid Hormone Secretion by Electrical Stimulation of Sympathetic and Parasympathetic Nerves in Anesthetized Rats. pubmed:28713236
Brain Research Age-Dependent Evaluation of Long-Term Depression Responses in Hyperthyroid Rats: Possible Roles of Oxidative Intracellular Redox Status Pubmed: 31288003
Journal of Physiological Sciences Thyroxin and calcitonin secretion into thyroid venous blood is regulated by pharyngeal mechanical stimulation in anesthetized rats Pubmed: 31270742
International Journal of Clinical and Experimental Medicine Apatinib inhibits proliferation of different lung cancer cells in vitro and in vivo by promoting apoptosis and inducing cell cycle arrest
Int J Dev Neurosci Effect of L©\thyroxine administration on long©\term potentiation and accompanying mitogen©\activated protein kinases in rats 33576121
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
IEA185Ge Instant ELISA Kit for Free Thyroxine (fT4) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA185Ge ELISA Kit for Free Thyroxine (fT4) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA185Ge CLIA Kit for Free Thyroxine (fT4) Chemiluminescent immunoassay for Antigen Detection.
LMA185Ge Multiplex Assay Kit for Free Thyroxine (fT4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.