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Active Semaphorin 3F (SEMA3F)

SEMA-IV; SEMA4; SEMAK; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3F

ACTIVITY TEST

Semaphorin-3F (SEMA3F) is a member of semaphorins family. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Semaphorins not only guide axons in development, but also have major roles in immune function (classes 4, 6, and 7) and the development of bones. Class 3 semaphorins are one of the most versatile semaphorin classes, in which Sema3a is the most studied. Besides, SEMA3F is deleted or downregulated in many metastatic tumors. To test the effect of SEMA3F on inhibit cell proliferation, human lung carcinoma cells A549 were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM prior to the addition of various concentrations of recombinant human SEMA3F. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of A549 cells after incubation with SEMA3F for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant SEMA3F for 48h. The result was shown in Figure 2. It was obvious that SEMA3F significantly increased cell viability of A549 cells. (A) A549 cells cultured in DMEM, stimulated with 100ng/mL SEMA3F for 48h; (B) Unstimulated A549 cells cultured in DMEM for 48h.
Figure. Cell proliferation of A549 cells inhibtion by stimulating with SEMA3F.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Molecular Neurobiology Reduced Sympathetic Innervation in Endometriosis is Associated to Semaphorin 3C and 3FExpression. pubmed:27558236
PLoS One Semaphorin 3F expression is reduced in pregnancy complicated by preeclampsia. An observational clinical study. pubmed:28350837
Journal of clinical investigation Semaphorin 3F signaling actively retains neutrophils at sites of inflammation Pubmed: 32191647
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APL930Hu01 Active Semaphorin 3F (SEMA3F) Cell culture; Activity Assays.
RPL930Hu01 Recombinant Semaphorin 3F (SEMA3F) Positive Control; Immunogen; SDS-PAGE; WB.
RPL930Hu02 Recombinant Semaphorin 3F (SEMA3F) Positive Control; Immunogen; SDS-PAGE; WB.
PAL930Hu02 Polyclonal Antibody to Semaphorin 3F (SEMA3F) WB; IHC; ICC; IP.
PAL930Hu01 Polyclonal Antibody to Semaphorin 3F (SEMA3F) WB; IHC; ICC; IP.
SEL930Hu ELISA Kit for Semaphorin 3F (SEMA3F) Enzyme-linked immunosorbent assay for Antigen Detection.
LML930Hu Multiplex Assay Kit for Semaphorin 3F (SEMA3F) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.