≡

Active Thymidylate Synthetase (TYMS)

TS; TMS; TSase; Thymidylate Synthase

ACTIVITY TEST

Thymidylate synthetase (TYMS) is an enzyme that catalyzes the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP). The enzyme is essential for regulating the balanced supply of the 4 DNA precursors in normal DNA replication: defects in the enzyme activity affecting the regulation process cause various biological and genetic abnormalities. Thymidylate synthase is an enzyme of about 30 to 35kDa in most species except in protozoan and plants where it exists as a bifunctional enzyme that includes a dihydrofolate reductase domain. Besides, Aspartate Aminotransferase (AST) has been identified as an interactor of TYMS, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TYMS and recombinant human AST. Briefly, TYMS were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to AST-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TYMS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TYMS and AST was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of TYMS with AST.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPG963Hu01 Recombinant Thymidylate Synthetase (TYMS) Positive Control; Immunogen; SDS-PAGE; WB.
APG963Hu01 Active Thymidylate Synthetase (TYMS) Cell culture; Activity Assays.
PAG963Hu01 Polyclonal Antibody to Thymidylate Synthetase (TYMS) WB,IHC
MAG963Hu21 Monoclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IP.
SEG963Hu ELISA Kit for Thymidylate Synthetase (TYMS) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG963Hu Multiplex Assay Kit for Thymidylate Synthetase (TYMS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSG963Hu01 ELISA Kit DIY Materials for Thymidylate Synthetase (TYMS) Main materials for "Do It(ELISA Kit) Yourself"