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Active Interleukin 28B (IL28B)

IFNL3; Interferon,Lambda 3; Cytokine Zcyto22

ACTIVITY TEST

Figure. The binding activity of IL28B with IL10Rb.
Interleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Journal of Antimicrobial Chemotherapy Impact of IL28B gene polymorphisms on interferon-λ3 plasma levels during pegylated interferon-α/ribavirin therapy for chronic hepatitis C in patients coinfected with HIV PubMed: 22294646
Cytokine Different effects of three interferons L on Toll-like receptor-related gene expression in HepG2 cells. Pubmed: 24041672
Hepatology Polymorphisms in melanoma differentiation‐associated gene 5 link protein function to clearance of hepatitis C virus Pubmed:25130193
Cytokine. Increased concentration of interferon lambda-3, interferon beta and interleukin-10 in the cerebrospinal fluid of patients with tick-borne encephalitis Pubmed:25461389
J Biomed Res.  Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 … pmc:PMC4726832
Journal of Biomedical Research Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 patients PMC4726832
LUPUS Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus Pubmed: 30336754
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APC028Hu01 Active Interleukin 28B (IL28B) Cell culture; Activity Assays.
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PAC028Hu01 Polyclonal Antibody to Interleukin 28B (IL28B) WB; IHC; ICC; IP.
LAC028Hu81 FITC-Linked Polyclonal Antibody to Interleukin 28B (IL28B) WB; IHC; ICC; IF.
LAC028Hu71 Biotin-Linked Polyclonal Antibody to Interleukin 28B (IL28B) WB; IHC; ICC.
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HEC028Hu High Sensitive ELISA Kit for Interleukin 28B (IL28B) Enzyme-linked immunosorbent assay for Antigen Detection.
SEC028Hu ELISA Kit for Interleukin 28B (IL28B) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC028Hu CLIA Kit for Interleukin 28B (IL28B) Chemiluminescent immunoassay for Antigen Detection.
LMC028Hu Multiplex Assay Kit for Interleukin 28B (IL28B) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.