Active Interleukin 28B (IL28B)

IFNL3; Interferon,Lambda 3; Cytokine Zcyto22

ACTIVITY TEST

Figure. The binding activity of IL28B with IL10Rb.
Interleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Antimicrobial Chemotherapy Impact of IL28B gene polymorphisms on interferon-λ3 plasma levels during pegylated interferon-α/ribavirin therapy for chronic hepatitis C in patients coinfected with HIV PubMed: 22294646
Cytokine Different effects of three interferons L on Toll-like receptor-related gene expression in HepG2 cells. Pubmed: 24041672
Hepatology Polymorphisms in melanoma differentiation‐associated gene 5 link protein function to clearance of hepatitis C virus Pubmed:25130193
Cytokine. Increased concentration of interferon lambda-3, interferon beta and interleukin-10 in the cerebrospinal fluid of patients with tick-borne encephalitis Pubmed:25461389
J Biomed Res.  Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 … pmc:PMC4726832
Journal of Biomedical Research Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 patients PMC4726832
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APC028Hu01 Active Interleukin 28B (IL28B) Cell culture; Activity Assays.
LAC028Hu81 FITC-Linked Polyclonal Antibody to Interleukin 28B (IL28B) WB; IHC; ICC; IF.
LAC028Hu71 Biotin-Linked Polyclonal Antibody to Interleukin 28B (IL28B) WB; IHC; ICC.
MAC028Hu22 Monoclonal Antibody to Interleukin 28B (IL28B) WB; IHC; ICC; IP.
HEC028Hu High Sensitive ELISA Kit for Interleukin 28B (IL28B) Enzyme-linked immunosorbent assay for Antigen Detection.
SEC028Hu ELISA Kit for Interleukin 28B (IL28B) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC028Hu CLIA Kit for Interleukin 28B (IL28B) Chemiluminescent immunoassay for Antigen Detection.