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Active Fibroblast Growth Factor 10 (FGF10)

Keratinocyte growth factor 2

ACTIVITY TEST

Fibroblast Growth Factor 10(FGF10)is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, Fibroblast Growth Factor Receptor 2 (FGFR2) has been identified as an interactor of FGF10, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF10 and recombinant human FGFR2. Briefly, FGF10 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to FGFR2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF10 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of FGF10 and FGFR2 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Inflammation Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair. pubmed:28540539
Journal of Molecular Neuroscience Dysregulation of Fibroblast Growth Factor 10 in the Peripheral Blood of Patients with Schizophrenia Pubmed: 31256336
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB882Hu01 Recombinant Fibroblast Growth Factor 10 (FGF10) Positive Control; Immunogen; SDS-PAGE; WB.
EPB882Hu61 Eukaryotic Fibroblast Growth Factor 10 (FGF10) Positive Control; Immunogen; SDS-PAGE; WB.
APB882Hu01 Active Fibroblast Growth Factor 10 (FGF10) Cell culture; Activity Assays.
PAB882Hu06 Polyclonal Antibody to Fibroblast Growth Factor 10 (FGF10) WB; IHC; ICC; IP.
PAB882Hu01 Polyclonal Antibody to Fibroblast Growth Factor 10 (FGF10) WB; IHC; ICC; IP.
MAB882Hu22 Monoclonal Antibody to Fibroblast Growth Factor 10 (FGF10) WB; IHC; ICC; IP.
SEB882Hu ELISA Kit for Fibroblast Growth Factor 10 (FGF10) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB882Hu CLIA Kit for Fibroblast Growth Factor 10 (FGF10) Chemiluminescent immunoassay for Antigen Detection.
LMB882Hu Multiplex Assay Kit for Fibroblast Growth Factor 10 (FGF10) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.