Packages (Simulation)
Image (II)
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Figure. SDS-PAGE
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Figure. Western Blot
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Active B-Cell Activating Factor (BAFF)
CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like
- Product No.APB686Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.9
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 187
US$ 468
US$ 936
US$ 2808
US$ 7020
For more details, please contact local distributors!
ACTIVITY TEST
Tumor necrosis factor ligand superfamily member 13B protein (TNFSF13B) also known as B-cell activating factor (BAFF) is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Besides, Integrin Beta 1 (ITGb1) has been identified as an interactor of TNFSF13B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNFSF13B and recombinant human ITGb1. Briefly, TNFSF13B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to ITGb1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TNFSF13B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFSF13B and ITGb1 was shown in Figure 1, the EC50 was 0.74~0.93 ug/ml.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| International Immunopharmacology | Expression and effects of B-lymphocyte stimulator and its receptors in T cell-mediated autoimmune arthritis Pubmed:25598291 |
| Int Immunopharmacol | BAFF and its receptors involved in the inflammation progress in adjuvant induced arthritis rats PubMed: 26688104 |
| Clinical Rheumatology | Metabolic syndrome in Sj PubMed: 26370103 |
| Transplantation | Calcineurin Inhibitor Minimization With Ixazomib, an Investigational Proteasome Inhibitor, for the Prevention of Antibody Mediated Rejection in a Preclinical Model. PubMed: 25919767 |
| Rheumatology | Cortical bone density and thickness alterations by high-resolution peripheral quantitative computed tomography: association with vertebral fractures in primary … rheumatology.kew332.abstract |
| Clinical Immunology | Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis Pubmed:30056130 |
| Gene | A Significant Decrease of BAFF, APRIL, and BAFF Receptors Following Mesenchymal Stem Cell Transplantation in Patients with Refractory Rheumatoid Arthritis Pubmed: 31935514 |
| american journal of physiology-renal physiology | The expression of XBP1s in B lymphocytes is critical for pristane-induced lupusnephritis in mice Pubmed: 32249615 |
| diagnostics | Interstitial Score and Concentrations of IL-4R¦Á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial?¡ 33924683 |