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Active Suppressors Of Cytokine Signaling 3 (SOCS3)

ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3

ACTIVITY TEST

Suppressor of cytokine signaling 3 (SOCS3) is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family that negatively regulates cytokine signal transduction. SOCS3 is feedback inhibitors of the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) signaling pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. SOCS3 also can bind to JAK2 kinase inhibits its kinase activity and nhibits insulin signaling in adipose tissue and the liver. Besides, Glycoprotein 130 (gp130) has been identified as an interactor of SOCS3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SOCS3 and recombinant mouse gp130. Briefly, SOCS3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SOCS3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SOCS3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of SOCS3 and gp130 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
The Journal of Biological Chemistry Human primary keratinocytes show a restricted ability to up-regulate suppressor of cytokine signaling (SOCS)3 compared to autologous macrophages Jcb: 280370
The Journal of Biological Chemistry Human Primary Keratinocytes Show Restricted Ability to Up-regulate Suppressor of Cytokine Signaling (SOCS)3 Protein Compared with Autologous Macrophages PubMed: PMC3323061
Journal of Reproductive Immunology What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? Pubmed: 24368037
The Journal of Immunology Alveolar Epithelial Cell–Derived Prostaglandin E2 Serves as a Request Signal for Macrophage Secretion of Suppressor of Cytokine Signaling 3 during Innate Inflammation Pubmed:27183597
Scientific Reports The FXR Agonist, Obeticholic Acid, Suppresses HCC Proliferation & Metastasis: Role of IL-6/STAT3 Signalling Pathway pubmed:28970500
Journal of Immunological Methods Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs. pubmed:29154772
Turkish Journal Of Medical Sciences The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility Pubmed:29914245
Novel trajectories of Bromocriptine antidiabetic action: Leptin-IL-6/JAK2/p-STAT3/SOCS3, p-IR/p-AKT/GLUT4, PPARγ/Adiponectin, Nrf2/PARP-1, and GLP-1 articles:10.3389
JCI Insight Alveolar macrophage secretion of vesicular SOCS3 represents a platform for lung cancer therapeutics Pubmed: 31619584
European Journal of Pharmacology Olmesartan attenuates type 2 diabetes-associated liver injury: Cross-talk of AGE/RAGE/JNK, STAT3/SCOS3 and RAS signaling pathways Pubmed: 32067934
FASEB JOURNAL Resident alveolar macrophage‐derived vesicular SOCS3 dampens allergic airway inflammation Pubmed: 32030817
molecular immunology SOCS3-deficient lung epithelial cells uptaking neutrophil-derived SOCS3 worsens lung influenza infection Pubmed: 32645550
Experimental and Therapeutic Medicine Changes and clinical significance of serum inflammatory factors in the treatment of pregnancy hypertension syndrome with magnesium sulfate combined with … Pubmed: 32742411
NEUROCHEMICAL RESEARCH Modulation of the Nitric Oxide/BH4 Pathway Protects Against Irradiation-Induced Neuronal Damage 33755856
BIOMEDICINE & PHARMACOTHERAPY Potential contribution of ELISA and LFI Assays to assessment of the oxidative stress condition based on 8-oxodG biomarker 33957564
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB684Mu02 Recombinant Suppressors Of Cytokine Signaling 3 (SOCS3) Positive Control; Immunogen; SDS-PAGE; WB.
RPB684Mu01 Recombinant Suppressors Of Cytokine Signaling 3 (SOCS3) Positive Control; Immunogen; SDS-PAGE; WB.
APB684Mu01 Active Suppressors Of Cytokine Signaling 3 (SOCS3) Cell culture; Activity Assays.
PAB684Mu02 Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3) WB; IHC; ICC; IP.
PAB684Mu01 Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3) WB; IHC; ICC; IP.
LAB684Mu71 Biotin-Linked Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3) WB; IHC; ICC.
LAB684Mu81 FITC-Linked Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3) WB; IHC; ICC; IF.
SEB684Mu ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB684Mu Multiplex Assay Kit for Suppressors Of Cytokine Signaling 3 (SOCS3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.