≡

Active Surfactant Protein C (SP-C)

SFTPC; PSP-C; SFTP2; SPC; SP5; Surfactant Associated Protein C; Pulmonary Surfactant Protein C; Pulmonary surfactant-associated proteolipid SPL(Val)

ACTIVITY TEST

Surfactant Associated Protein C (SPC) is one of the pulmonary surfactant proteins. It is a membrane protein which manufactures surfactant. The propeptide of pulmonary surfactant C has an N-terminal alpha-helical segment whose suggested function was stabilization of the protein structure, since the latter can irreversibly transform from its native alpha-helical structure to beta-sheet aggregates and form amyloid fibrils. Besides, Monokine Induced By Interferon Gamma (MIg) has been identified as an interactor of SPC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SPC and recombinant rat MIg. Briefly, SPC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to MIg-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SPC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SPC and MIg was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of SPC with MIg.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
American Journal of Rhinology & Allergy Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps PubMed: 23406594
Plos one Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins PubMed: PMC3551896
Plos one The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS PubMed: PMC3787032
Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge Opus4:Source
Mol Cell Biochem Autophagy regulates hyperoxia-induced intracellular accumulation of surfactant protein C in alveolar type II cells PubMed: 26122393
Plos One Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) Pubmed:27337142
PLoS One The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions. pubmed:27656877
Frontiers in Aging Neuroscience Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid. pubmed:28101052
Frontiers in Aging Neuroscience Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid PMC5209370
American Journal of Physiology. Lung Cellular and Molecular Physiology Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. pubmed:29167126
Respiratory research Effects and molecular mechanisms of intrauterine infection/inflammation on lung development Pubmed:29747649
Lung  Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation Pubmed:29445934
Molecular Neurobiology Rheologically Essential Surfactant Proteins of the CSF Interacting with Periventricular White Matter Changes in Hydrocephalus Patients–Implications for CSF … Doi: 10.1007/s12035-019-01648-z
Stem Cells International Adipose Tissue-Derived Stem Cells Have the Ability to Differentiate into Alveolar Epithelial Cells and Ameliorate Lung Injury Caused by Elastase-Induced Emphysema …
Stem Cells International Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor Pubmed: 31949433
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APB623Ra01 Active Surfactant Protein C (SP-C) Cell culture; Activity Assays.
RPB623Ra01 Recombinant Surfactant Protein C (SP-C) Positive Control; Immunogen; SDS-PAGE; WB.
RPB623Ra02 Recombinant Surfactant Protein C (SP-C) Positive Control; Immunogen; SDS-PAGE; WB.
PAB623Ra01 Polyclonal Antibody to Surfactant Protein C (SP-C) WB; IHC; ICC; IP.
PAB623Ra02 Polyclonal Antibody to Surfactant Protein C (SP-C) WB; IHC; ICC; IP.
MAB623Ra21 Monoclonal Antibody to Surfactant Protein C (SP-C) WB; IHC; ICC; IP.
SEB623Ra ELISA Kit for Surfactant Protein C (SP-C) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB623Ra Multiplex Assay Kit for Surfactant Protein C (SP-C) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.