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Active Interleukin 7 (IL7)

Pre-B-cell Growth Factor; Lymphopoietin-1

ACTIVITY TEST

IL7 (Interleukin 7) is a hematopoietic growth factor secreted by stromal cells in the bone marrow and thymus. The interaction between IL17 and the IL7 receptor triggers a cascade of signals important for T-cell development within the thymus and survival within the periphery. It is reported that IL-7 acts on both resting and activated T cells, including Jurkat cells. Thus, a proliferation assay of recombinant human IL7 was conducted using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL7. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL7 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL7 significantly promoted cell proliferation of Jurkat cells.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Experimental Gerontology Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia Pubmed:30071285
Cardiovascular Therapeutics Role of IL‐7 in Fusion of Rat Bone Marrow Mesenchymal Stem Cells with Cardiomyocytes in vitro and Improvement of Cardiac Function in vivo Pubmed: 30451388
Stem Cells and Development The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymus Pubmed: 30511904
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA662Hu01 Active Interleukin 7 (IL7) Cell culture; Activity Assays.
APA662Hu61 Active Interleukin 7 (IL7) Cell culture; Activity Assays.
RPA662Hu01 Recombinant Interleukin 7 (IL7) Positive Control; Immunogen; SDS-PAGE; WB.
EPA662Hu61 Eukaryotic Interleukin 7 (IL7) Positive Control; Immunogen; SDS-PAGE; WB.
PAA662Hu01 Polyclonal Antibody to Interleukin 7 (IL7) WB; IHC; ICC; IP.
MAA662Hu29 Monoclonal Antibody to Interleukin 7 (IL7) WB; IHC; ICC; IP.
MAA662Hu22 Monoclonal Antibody to Interleukin 7 (IL7) WB; IHC; ICC; IP.
MAA662Hu21 Monoclonal Antibody to Interleukin 7 (IL7) WB; IHC; ICC; IP.
MAA662Hu23 Monoclonal Antibody to Interleukin 7 (IL7) WB
MAA662Hu24 Monoclonal Antibody to Interleukin 7 (IL7) WB; IHC; ICC; IP.
MAA662Hu25 Monoclonal Antibody to Interleukin 7 (IL7) WB; IHC; ICC; IP.
MAA662Hu27 Monoclonal Antibody to Interleukin 7 (IL7) WB; IHC; ICC; IP.
MEA662Hu Mini Samples ELISA Kit for Interleukin 7 (IL7) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA662Hu ELISA Kit for Interleukin 7 (IL7) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA662Hu Multiplex Assay Kit for Interleukin 7 (IL7) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.