Active Amiloride Binding Protein 1 (ABP1)
AOC1; DAO; KAO; Histaminase; Kidney amine oxidase; Diamine Oxidase; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1
- Product No.APA656Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point6.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 301
For more details, please contact local distributors! US$ 752 US$ 1504 US$ 4512 US$ 11280
ACTIVITY TEST
Figure. The binding activity of DAO with DDC.
Diamine oxidase (DAO), also known as histaminase, is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. It is also secreted by eosinophils. In case of a shortage of diamine oxidase in the human body, it may appear as an allergy or histamine intolerance. Besides, Dopa Decarboxylase (DDC) has been identified as an interactor of DAO, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DAO and recombinant human DDC. Briefly, DAO were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to DDC-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DAO pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DAO and DDC was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE
Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine | Citations |
Applied Clay Science | Effects of copper-exchanged montmorillonite, as alternative to antibiotic, on diarrhea, intestinal permeability and proinflammatory cytokine of weanling pigs Cas.zju: Source |
Phytomedicine.? | A multi-component herbal preparation, STW 5, shows anti-apoptotic effects in radiation induced intestinal mucositis in rats Pubmed:25022208 |
Oxid Med Cell Longev | Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation Model PubMed: 26064429 |
Pancreatology. | Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. Pubmed:26804005 |
Biochimica et Biophysica Acta (BBA)-General Subjects | Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats pubmed:28341485 |
Journal of Controlled Release | A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury pubmed:28522195 |
Advances in Experimental Medicine and Biology | Serum Diamine Oxidase in Pseudoallergy in the Pediatric Population pubmed:28804811 |
PLoS One | Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats pubmed:28622357 |
Nutrients | Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron Pubmed:29861488 |
Scientific Reports | Pregnancy-associated diamine oxidase originates from extravillous trophoblasts and is decreased in early-onset preeclampsia Pubmed:29679053 |
British Journal of Nutrition | Postoperative symbiotic in patients with head and neck cancer: a double-blind randomised trial Pubmed:29277158 |
Food & Function | The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer Pubmed:29384543 |
硕士论文 | LOCALIZATION OF COPPER-CONTAINING AMINE OXIDASE ENZYMES IN THE BLOOD |
International Journal of Clinical and Experimental Medicine | Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease |
Journal of Functional Foods | Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease |
Acta Diabetologica | Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated mice Pubmed: 31749050 |
Biomed Research International | Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet Pubmed: 32149099 |
FASEB JOURNAL | HMGB1‐associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in rats Pubmed: 31961020 |
JOURNAL OF ETHNOPHARMACOLOGY | The effect of Poria cocos ethanol extract on the intestinal barrier function and intestinal microbiota in mice with breast cancer Pubmed: 33039631 |
Cell Death Discovery | Fecal microbiota transplantation from patients with autoimmune encephalitis modulates Th17 response and relevant behaviors in mice Pubmed: 32821439 |
The Journal of Nutritional Biochemistry | Effects of fucoidan on tumor prevention and gut flora |
Chemical Biology & Dug Design | Design and development of novel fasudil derivatives as potent anti‐breast cancer agent that improves intestinal flora and intestinal barrier function in rats 34587363 |
Journal of Functional Foods | Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways |
Stem Cell Research & Therapy | Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model |