Active Epidermal Growth Factor (EGF)

URG; Beta-Urogastrone; Pro-epidermal growth factor

ACTIVITY TEST

Figure. Cell proliferation of 3T3 cells after stimulated with EGF.
Epidermal growth factor (EGF) binding to EGFR, results in cellular proliferation, differentiation, and survival. To test the effect of EGF on cell proliferation, 3T3 fibroblasts cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM prior to the addition of various concentrations of recombinant mouse EGF. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of 3T3 cells after incubation with EGF for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant EGF for 96h. The result was shown in Figure 2. It was obvious that EGF significantly increased cell viability of 3T3 cells.
(A) 3T3 cells cultured in DMEM, stimulated with 1ng/mL EGF for 96h;
(B) Unstimulated 3T3 cells cultured in DMEM for 96h.

Figure. Cell proliferation of 3T3 cells after stimulated with EGF.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Pediatric Surgery The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model PubMed: 20438940
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism PubMed: PMC3623607
Plos one The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor PubMed: PMC3590171
Diabetes. Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity Pubmed:25795216
Eur Rev Med Pharmacol Sci.? Association of colon adenomas and skin tags: coincidence or coexistence? Pubmed:24763889
Int J Clin Exp Med. The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972
ACS Applied Materials & Interfaces Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness. pubmed:27709887
Canadian Journal of Physiology and Pharmacology The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats pubmed:28177680
Journal of Controlled Release A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury pubmed:28522195
Scientific Reports Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets pubmed:28811665
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA560Mu02 Recombinant Epidermal Growth Factor (EGF) Positive Control; Immunogen; SDS-PAGE; WB.
APA560Mu01 Active Epidermal Growth Factor (EGF) Cell culture; Activity Assays.
RPA560Mu01 Recombinant Epidermal Growth Factor (EGF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA560Mu02 Polyclonal Antibody to Epidermal Growth Factor (EGF) WB; IHC; ICC; IP.
PAA560Mu01 Polyclonal Antibody to Epidermal Growth Factor (EGF) WB; IHC; ICC; IP.
SEA560Mu ELISA Kit for Epidermal Growth Factor (EGF) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA560Mu Mini Samples ELISA Kit for Epidermal Growth Factor (EGF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA560Mu CLIA Kit for Epidermal Growth Factor (EGF) Chemiluminescent immunoassay for Antigen Detection.