Active Fibroblast Growth Factor 2, Basic (FGF2)
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
- Product No.APA551Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point9.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors! US$ 540
For more details, please contact local distributors! US$ 1080
For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors! US$ 8100
For more details, please contact local distributors!
Figure. The binding activity of FGF2 with CASP1.
Basic fibroblast growth factor (FGF2), also known as bFGF, FGF-β is a member of a large family of structurally related heparin-binding proteins (the FGFs) involved in the regulation of cell proliferation, growth and differentiation. It involved in many biological processes including angiogenesis, embryonic development and wound healing. Additionally, FGF2 is a critical component of human embryonic stem cell culture medium. Besides, Caspase 1 (CASP1) has been identified as an interactor of FGF2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF2 and recombinant human CASP1. Briefly, FGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CASP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FGF2 and CASP1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Molecular Mass Marker for Protein
- Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
- Real Time PCR Experimental Service
- BCA Protein Quantification Kit
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- PBS Buffer
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
|Journal of Pediatric Surgery||The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model PubMed: 20438940|
|International Journal of Nanomedicine||Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis PubMed: PMC3254262|
|Archives of Animal Nutrition||Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesis Tandfonline: 644918|
|Diabetes||Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb Ischemia Pubmed: 22266669|
|Molecular Pharmacology||Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic Rats Pubmed: 22091745|
|Science Alert||Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats Scialert: Source|
|Nanoscale Research Letters||Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart. PubMed: 22827927|
|PLoS One||Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis PubMed: PMC3771850|
|International Journal of Molecular Sciences||Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles NCBI:PMC3856104|
|Archives of Animal Nutrition||Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesis Pubmed: 23952606|
|Journal of Rehabilitation Research & Development||Role of sensory and motor intensity of electrical stimulation on fibroblastic growth factor-2 expression, inflammation, vascularization, and mechanical strength of full-thickness wounds Pubmed: 23934870|
|PLoS One.?||Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate Neurotoxicity Pubmed:25426952|
|Implant Dentistry||Effects of Hemostatic Agents on Fibroblast Cells Lww:Source|
|Journal of Materials Chemistry B||bFGF-grafted electrospun fibrous scaffolds via poly(dopamine) for skin wound healing Rsc:Source|
|Archives of Animal Nutrition||Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos Pubmed:25530495|
|Int J Clin Exp Med.||The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972|
|Theriogenology||Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes Science: Article|
|Tumor Biology||Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151|
|Acta Biomaterialia||Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosa article:S1742706115301410|
|22||Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars Pubmed:26774564|
|Genet Mol Res||Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064|
|BMC Neuroscience||3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPA pubmed:27456338|
|Medical Science Monitor||5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts 898221|
|Biomedicine & Pharmacotherapy||FGF-2-mediated FGFR1 signaling in human microvascular endothelial cells is activated by vaccarin to promote angiogenesis pubmed:28841454|
|Medical Science Monitor||5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts pubmed:28052053|
|International Journal of Clinical and Experimental Pathology||Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424|
|Journal of Biomaterials and Tissue Engineering||The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice 10.1166/jbt.2017.1652|