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Active Growth Differentiation Factor 9 (GDF9)

ACTIVITY TEST

GDF9 (Growth/differentiation factor 9) is an oocyte derived growth factora which belongs to the transforming growth factor-beta (TGFβ) superfamily. GDF9 is required for ovarian folliculogenesis and promotes primordial follicle development. S100A8 has been identified as an interactor of GDF9 through two-hybrid assay, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GDF9 and recombinant human S100A8. Briefly, GDF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A8-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GDF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of GDF9 and S100A8 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Women's medicine Follicular fluid growth differentiation factor-9 concentration and oocyte developmental competence Ejwm: 0637JWM
Journal of Ovarian Research Dehydroepiandrosterone improves follicular fluid bone morphogenetic protein-16 and accumulated embryo score of infertility patients with diminished ovarian reserve undergoing in vitro fertilization: a randomized controlled trial Pubmed:25330837
Oncotarget Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy Pubmed:29423092
Development Oocytes and hypoxanthine orchestrate the G2-M switch mechanism in ovarian granulosa cells Pubmed: 32620578
Reprod Sci Compromised Cumulus-Oocyte Complex Matrix Organization and Expansion in Women with PCOS 34748173
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA427Hu01 Recombinant Growth Differentiation Factor 9 (GDF9) Positive Control; Immunogen; SDS-PAGE; WB.
APA427Hu01 Active Growth Differentiation Factor 9 (GDF9) Cell culture; Activity Assays.
PAA427Hu01 Polyclonal Antibody to Growth Differentiation Factor 9 (GDF9) WB; IHC; ICC; IP.
MAA427Hu22 Monoclonal Antibody to Growth Differentiation Factor 9 (GDF9) WB; IHC; ICC; IP.
SEA427Hu ELISA Kit for Growth Differentiation Factor 9 (GDF9) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA427Hu Multiplex Assay Kit for Growth Differentiation Factor 9 (GDF9) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.