Active Anti-Mullerian Hormone (AMH)

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

ACTIVITY TEST

Anti-Müllerian hormone (AMH), also named Müllerian inhibiting substance (MIS) belongs to a tissue-specific TGF-beta superfamily growth factor. It can be expressed by male sertoli cells and postnatal testis, and ovarian granulosa cells of females postpartum. AMH expression is critical to sex differentiation at a specific time during fetal development, it appears to be tightly regulated by SF1, GATA factors, DAX1 and FSH. AMH signals through a characteristic receptor consisting of a type I and a type II receptor serine/threonine kinase. Especially the type II receptor is unique and specific receptor for AMH. Besides, Mothers Against Decapentaplegic Homolog 9 (Smad9) has been identified as an interactor of AMH, thus a binding ELISA assay was conducted to detect the interaction of recombinant human AMH and recombinant human (Smad9) Briefly, AMH were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Smad9-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AMH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of AMH and Smad9 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Journal of Zhejiang University SCIENCE B Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice. PubMed: 23125082
In Vivo.(Athens,Greece) Association Between AMH, Oocyte Number and Availability of Embryos for Cryopreservation in IVF Pubmed: 24292596
Gynecological Endocrinology The effects of isotretinoin on the ovarian reserve of females with acne Informahealthcare: Source
Fertility and Sterility Increased expression of anti mullerian hormone and its receptor in endometriosis Ingentaconnect: Source
Reproductive BioMedicine Online Decreased ovarian reserve in female Sprague–Dawley rats induced by isotretinoin (retinoic acid) exposure Rbmojournal: Source
Reproductive Biology and Endocrinology The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study. Pubmed: 24341292
Biomed Res Int. Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats Pubmed:Pmc4053297
Fertil Steril. Increased expression of antimüllerian hormone and its receptor in endometriosis Pubmed:24613539
The Journal of Maternal-Fetal & Neonatal Medicine Maternal serum anti-Müllerian hormone at 11-13 weeks' gestation in the prediction of preeclampsia Pubmed:24953352
J Gynecol Oncol. Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model Pubmed:25142624
Reproductive BioMedicine Online The effect of childhood cancer therapy on ovarian reserve and pubertal development Pubmed:25498597
Reproductive Sciences Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome Pubmed:25667201
Yonsei Medical Journal Anti-Proliferative and Apoptotic Activities of Müllerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines PubMed: 26632380
oncology research Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940
Reprod Biomed Online Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats PubMed: 26206282
PLoS One Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis PubMed: 26465611
Toxicol Lett Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood PubMed: 26657179
Journal of Assisted Reproduction and Genetics Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage? Pubmed:27255570
Anticancer Res. Prognostic Value of Anti-Müllerian Hormone and Inhibin B in Patients with Premenopausal Hormone Receptor-positive Breast Cancer Pubmed:26976997
Chemosphere. Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. pubmed:27567155
Theriogenology Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males. pubmed:28043339
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061
Journal of Assisted Reproduction and Genetics Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. pubmed:28074436
Journal Pakistan Medical Association Serum anti-mullerian hormone: Correlation with the ovarian follicular dynamics in healthy mice pubmed:27654725
Advance Medical Associates Are anti-Müllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes? pubmed:27162065
Yonsei Med J Volume Anti-Proliferative and Apoptotic Activities of M? llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines. pubmed:26632380
Journal of Endocrinological Investigation The use of serum anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) to predictthe number of oocytes collected and availability of embryos for cryopreservation in IVF. pubmed:27465668
Journal of the Pakistan Medical Association Predictive accuracy of anti mullerian hormone as indicator of ovarian follicle loss in cyclophosphamide treated mice pubmed:28955058
International Journal of Reproduction, Contraception, Obstetrics and Gynecology Can Co-enzyme Q10 improve the chances of conception after the age of 35? 10.18203/2320-1770.ijrcog20172902
Animal Production Science Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? 10.1071/ANv57n12Ab065
Reproduction. Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593
Theriogenology Relationship among circulating anti-Müllerian hormone, insulin like growth factor 1, cadmium and superovulatory response in dairy cows. pubmed:28708536
Journal of ovarian research Rapamycin Prevents cyclophosphamide-induced Over-activation of Primordial Follicle pool through PI3K/Akt/mTOR Signaling Pathway in vivo. pubmed:28814333
Annals of Rheumatic Diseases  FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study Suppl_2:728.1.abstract
Free Radical Biology & Medicine Peroxiredoxin 2 deficiency accelerates age-related ovarian failure through the reactive oxygen species-mediated JNK pathway in mice Pubmed:29778464
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625
Biology of Reproduction Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss Pubmed:29351580
Endocrine Connections Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery Pubmed:30012648
Reproductive BioMedicine Online Dienogest suppresses the activation of primordial follicles and preserves the primordial follicle stockpile for fertility in mice Pubmed:29609766
Journal of Ovarian Research Comparison of the influence of three fibroid treatment options: supracervical hysterectomy, ulipristal acetate and uterine artery embolization on ovarian … Pubmed:29859107
Animal Reproduction Science Anti-Müllerian hormone and Oestradiol as markers of future reproductive success in juvenile gilts Pubmed:29859701
Stem Cell Research & Therapy Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243
Biochemical and Biophysical Research Communications Involvement of obesity-associated upregulation of chemerin/chemokine-like receptor 1 in oxidative stress and apoptosis in ovaries and granulosa cells Pubmed: 30722991
Lupus Levels of anti-Müllerian hormone in premenopausal women with the antiphospholipid syndrome and its association with the risk of clinical complications Pubmed: 30717622
In Vitro Cellular & Developmental Biology - Animal Transplantation of umbilical cord–derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice Pubmed: 30820812
Biomedicine & Pharmacotherapy Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve
Reproductive Biology and Endocrinology Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … Pubmed: 31311554
Oxidative Medicine and Cellular Longevity Carvacrol and Thymol Modulate the Cross-Talk between TNF-α and IGF-1 Signaling in Radiotherapy-Induced Ovarian Failure
Acta Obstet Gynecol Scand The impact of uterine artery embolization on ovarian reserve: a systematic review and meta‐analysis Pubmed: 31370100
Reproductive Sciences Uterine Cells Improved Ovarian Function in a Murine Model of Ovarian Insufficiency Pubmed: 31530098
Animals Serum Concentrations of AMH and E2 and Ovarian and Uterine Traits in Gilts Pubmed: 31619004
Journal Of Pakistan Medical Association The effect of acupuncture on anti-mullerian hormone and assisted reproduction outcome in Polycystic Ovary Syndrome patients undergoing in vitro fertilization … Pubmed: 31603867
In Vitro Cellular & Developmental Biology Basal characterization and in vitro differentiation of putative stem cells derived from the adult mouse ovary Pubmed: 31900800
J Assist Reprod Genet The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage Pubmed: 32020412
Biology (Basel). Seminal Plasma Anti-Müllerian Hormone: A Potential AI-Boar Fertility Biomarker? Pubmed: 32290279
Eur J Breast Health The Effect of Systemic Chemotherapy on Ovarian Function: A Prospective Clinical Trial Pubmed: 32656517
Heliyon Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting … Pubmed: 33005806
CYTOMETRY PART A After cyclophosphamide exposure, granulosa cells recover their anti©\M¨¹llerian hormone©\producing ability but not their numbers 33342073
BIOLOGY OF REPRODUCTION Igf3 is essential for ovary differentiation in zebrafish 33276384
JOURNAL OF ETHNOPHARMACOLOGY Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis 33422655
Stem Cells Int Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … 34733331
Sci Total Environ Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression Pubmed:35276145
Hum Reprod Update Anti-Müllerian hormone as a marker of ovarian reserve and premature ovarian insufficiency in children and women with cancer: a systematic review Pubmed:35199161
Horm Metab Res Anti-Mullerian Hormone Levels Increase After Bariatric Surgery in Obese Female Patients With and Without Polycystic Ovary Syndrome Pubmed:35276745
American Journal of Translational Research The effects of vasopressin injection technique on ovarian reserve in laparoscopic cystectomy of bilateral ovarian endometrioma: a retrospective cohort study Pubmed:35559399
Stem Cell Research & Therapy G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and …
Research Square Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency
Fundamental Research Long-term amelioration of an early-onset familial atrial fibrillation model with AAV-mediated in vivo gene therapy
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