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Active Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)

SFD; TIMP Metallopeptidase Inhibitor 3; Metallopeptidase Inhibitor 3; Sorsby Fundus Dystrophy; Pseudoinflammatory

ACTIVITY TEST

Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) is an protein belongs to the tissue inhibitor of metalloproteinases family. They are inhibitors of the matrix metalloproteinases. TIMP-3 is the only member of the TIMP family which is found exclusively in the extracellular matrix (ECM). It is regulated in a cell cycle-dependent fashion in certain cell types and may serve as a marker for terminal differentiation. The activity of recombinant human TIMP3 was measured by its ability to inhibit recombinant human MMP2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (v/v), pH 7.5. The rhMMP-2 (APA100Hu61) was activated with 1 mM APMA at 37 °C for 1h. Then 16 μl 126 ug/mL rhTIMP-3, 25.6 µL of 100 ug/ml rhMMP-2 and 118.4 µL of assay buffer was incubated for 2 hours at 37 °C, including a control (in duplicate) containing assay buffer and the diluted rhMMP-2. The mixtures was diluted 5 fold in assay buffer followed by adding 50 ul 20 uM substrate, including a control containing assay buffer and substrate. Then read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively in kinetic mode for 5 minutes. Under these conditions, the enzyme amount of 50% inhibition of rhMMP-2 activity per minute is defined as a unit. The specific activity of TIMP3 is >6000 U/mg.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Neuroimmunomodulation Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus Pubmed: source
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Physiol. Res. The unwounded skin remodeling in animal models of diabetes types 1 and 2. Pubmed: 24020818
VirusDisease Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection Pubmed:26925446
Acta Pharmacol Sin. Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. pubmed:27890915
Biochemical genetics Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer pubmed:28421310
International Journal of Cancer Circulating and tissue IMP3 levels are correlated with poor survival in renal cell carcinoma Pubmed: 30650187
J. Pers. Med Altered TIMP-3 Levels in the Cerebrospinal Fluid and Plasma of Patients with Alzheimer's Disease Pubmed:35629249
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA129Hu01 Recombinant Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) Positive Control; Immunogen; SDS-PAGE; WB.
APA129Hu01 Active Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) Cell culture; Activity Assays.
PAA129Hu01 Polyclonal Antibody to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) WB; IHC; ICC; IP.
MAA129Hu21 Monoclonal Antibody to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) WB; IHC; ICC; IP.
MAA129Hu22 Monoclonal Antibody to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) WB; IHC; ICC; IP.
SEA129Hu ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA129Hu CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) Chemiluminescent immunoassay for Antigen Detection.
LMA129Hu Multiplex Assay Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.