Active Stromal Cell Derived Factor 1 (SDF1)
CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12
- Product No.APA122Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point8.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors! US$ 660
For more details, please contact local distributors! US$ 1320
For more details, please contact local distributors! US$ 3960
For more details, please contact local distributors! US$ 9900
For more details, please contact local distributors!
Figure. The binding activity of SDF1 with CCR1
The stromal cell-derived factor 1 (SDF1), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein. SDF1 is strongly chemotactic for lymphocytes. During embryogenesis, it directs the migration of hematopoietic cells from fetal liver to bone marrow and the formation of large blood vessels. CXCL12 is also chemotactic for mesenchymal stem cells and is expressed in the area of inflammatory bone destruction, where it mediates their suppressive effect on osteoclastogenesis. Besides, Chemokine C-C-Motif Receptor 1 (CCR1) has been identified as an interactor of SDF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SDF1 and recombinant rat CCR1. Briefly, SDF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to CCR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SDF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SDF1 and CCR1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Critical Care||Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD) Goescholar: source|
|22||The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels ScienceDirect: S0142961209012654|
|22||The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanisms ScienceDirect: S0142961211011422|
|Phytomedicine||Tanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia model ScienceDirect: S0944711310003260|
|Graefe's Archive for Clinical and Experimental Ophthalmology||Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cells PubMed: 21655971|
|Molecular Therapy||Pleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemia PubMed: 21224834|
|Oncogene||Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069|
|International Journal of Nephrology||Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis PubMed: 22792461|
|Rheumatology International||Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186|
|Experimental Eye Research||Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cells Pubmed: 24780853|
|Atherosclerosis||The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial Pubmed:24530788|
|International Journal of Cardiology||Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men Pubmed:24286591|
|Tissue Engineering Part A||Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat Pubmed:24814095|
|Pharmacol Rep.?||Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression Pubmed:25150002|
|Journal of Stroke and Cerebrovascular Diseases||Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke Patients Pubmed:25444027|
|Pharmacological Reports||The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders Pubmed:25560580|
|In Vitro Cellular & Developmental Biology - Animal||Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells Pubmed:25636237|
|Pharmacological Reports||Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder PubMed: 26481549|
|Biochem Biophys Res Commun||Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats PubMed: 26392311|
|Am J Transl Res||Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats PubMed: 26396666|
|Medicine (Baltimore)||A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion PubMed: 26632727|
|Pharmacological Reports||A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity Pubmed:27208878|
|Frontiers in Psychiatry||Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity. pubmed:28149283|
|European Journal of Applied Physiology||High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cellsand depressed shedding of vascular endothelial cells undergoing hypoxia. pubmed:27761657|
|Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease||AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungs pubmed:29233725|
|International Journal of Clinical and Experimental Pathology||Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424|
|BioMed Research International.||c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer pubmed:28573141|
|Scientific Reports||Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms. pubmed:28842629|
|Biomaterials||Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study. pubmed:29017079|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
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