Active Stromal Cell Derived Factor 1 (SDF1)

CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

ACTIVITY TEST

Figure. The binding activity of SDF1 with CCR1
The stromal cell-derived factor 1 (SDF1), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein. SDF1 is strongly chemotactic for lymphocytes. During embryogenesis, it directs the migration of hematopoietic cells from fetal liver to bone marrow and the formation of large blood vessels. CXCL12 is also chemotactic for mesenchymal stem cells and is expressed in the area of inflammatory bone destruction, where it mediates their suppressive effect on osteoclastogenesis. Besides, Chemokine C-C-Motif Receptor 1 (CCR1) has been identified as an interactor of SDF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SDF1 and recombinant rat CCR1. Briefly, SDF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to CCR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SDF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SDF1 and CCR1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Critical Care Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD) Goescholar: source
22 The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels ScienceDirect: S0142961209012654
22 The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanisms ScienceDirect: S0142961211011422
Phytomedicine Tanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia model ScienceDirect: S0944711310003260
Graefe's Archive for Clinical and Experimental Ophthalmology Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cells PubMed: 21655971
Molecular Therapy Pleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemia PubMed: 21224834
Oncogene Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069
International Journal of Nephrology Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis PubMed: 22792461
Rheumatology International Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186
Experimental Eye Research Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cells Pubmed: 24780853
Atherosclerosis The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial Pubmed:24530788
International Journal of Cardiology Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men Pubmed:24286591
Tissue Engineering Part A Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat Pubmed:24814095
Pharmacol Rep.? Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression Pubmed:25150002
Journal of Stroke and Cerebrovascular Diseases Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke Patients Pubmed:25444027
Pharmacological Reports The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders Pubmed:25560580
In Vitro Cellular & Developmental Biology - Animal Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells Pubmed:25636237
Pharmacological Reports Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder PubMed: 26481549
Biochem Biophys Res Commun Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats PubMed: 26392311
Am J Transl Res Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats PubMed: 26396666
Medicine (Baltimore) A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion PubMed: 26632727
Pharmacological Reports A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity Pubmed:27208878
Frontiers in Psychiatry Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity. pubmed:28149283
European Journal of Applied Physiology High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cellsand depressed shedding of vascular endothelial cells undergoing hypoxia. pubmed:27761657
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungs pubmed:29233725
International Journal of Clinical and Experimental Pathology Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424
BioMed Research International. c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer pubmed:28573141
Scientific Reports Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms. pubmed:28842629
Biomaterials Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study. pubmed:29017079
Biomaterials Science The effects of Sr-incorporated micro/nano rough titanium surface on rBMSC migration and osteogenic differentiation for rapid osteointegration Pubmed:29850672
International Journal of Molecular Medicine Low‑intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1‑mediated SDF‑1 expression Pubmed:29620151
Cardiovascular Drugs and Therapy β‐blockers interfere with cell homing receptors and regulatory proteins in a model of spontaneously hypertensive rats Pubmed:29752864
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