Active Interleukin 18 (IL18)

IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma

ACTIVITY TEST

Figure. The binding activity of IL18 with CASP1.
Interleukin-18 (IL18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. IL18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). Besides, Caspase 1 (CASP1) has been identified as an interactor of IL18, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL18 and recombinant human CASP1. Briefly, IL18 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CASP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL18 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL18 and CASP1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
International Immunopharmacology Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuries ScienceDirect: S1567576911001068
Atherosclerosis IL-18 overexpression promotes vascular inflammation and remodeling in a rat model of metabolic syndrome ScienceDirect: S0021915009006200
PLoS One. HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis Plos: Source
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Cell Death Dis. MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy Pubmed:Pmc4237254
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion Pubmed:24816434
J Korean Med Sci. Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart Disease Pubmed:25120320
PLOS ONE Protective Effect of Leaf Essential Oil from PubMed: 25794175
Fertil Steril Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” PubMed: 26474737
United States Patent Application GLUCAN-ENCAPSULATED SIRNA FOR TREATING TYPE 2 DIABETES MELLITUS y2016:0138028.html
Front Pharmacol Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata pubmed:27803670
Pharmacological reports The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder science:article
International Journal of Molecular Sciences PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2 pubmed:27973457
Cellular Physiology and Biochemistry MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a andRegulating Renal Tubular Cell Pyroptosis. pubmed:28006785
Journal of Food and Drug Analysis Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced … S1021-9498(17)30081-9
Journal of Microbiology, Immunology and Infection Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients pubmed:28655573
Discovery and Validation of Immunological Biomarkers in Milk for Health Monitoring of Dairy Cows - Results from a Multiomics Approach doi:10.4172/2329-888X.1000182
Cellular Physiology and Biochemistry Dexmedetomidine Alleviates HyperoxiaInduced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation pubmed:28873369
20 Melatonin prevents endothelial cell pyroptosis via regulation of long noncoding RNA MEG3/miR-223/NLRP3 axis pubmed:29024030
Pharmacological Reports The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. pubmed:28623707
World journal of gastroenterology Hypothermic machine perfusion with metformin-University of Wisconsin solution for ex vivo preservation of standard and marginal liver grafts in a rat model. pubmed:29142469
Veterinary microbiology Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses. pubmed:28532803
International immunopharmacology Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro pubmed:28605710
Journal of Neuroinflammation Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage. pubmed:28732502
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