Active Interleukin 15 (IL15)
- Product No.APA061Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point5.1
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 400
For more details, please contact local distributors! US$ 1000
For more details, please contact local distributors! US$ 2000
For more details, please contact local distributors! US$ 6000
For more details, please contact local distributors! US$ 15000
For more details, please contact local distributors!
ACTIVITY TEST
Interleukin 15 (IL15) is a widely expressed cytokine that is structurally and functionally related to IL2, which plays an important role in many immunological diseases. IL15 also regulates T and natural killer (NK) cell activation and proliferation. To test the effect of IL15 on cells proliferation of human T lymphocyte cells, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL15. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating
the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL15 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of IL15 was shown in Figure 2. It was obvious that IL15 significantly promoted cell proliferation of Jurkat cells. The ED50 for this effect is typically 0.7240 to 5.206ng/mL.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
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Magazine | Citations |
Endocrine | Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training Pubmed: 24006180 |
Endocrine | Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype Pubmed:25522723 |
Med Sci Moni | miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice PubMed: 26087886 |
Exercise physiology | Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle Dspace: Handle |
Biotechnology Letters | Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice s10529-017-2342-1 |
Journal of Controlled Release | Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. pubmed:28408199 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
APA061Hu01 | Active Interleukin 15 (IL15) | Cell culture; Activity Assays. |
RPA061Hu01 | Recombinant Interleukin 15 (IL15) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA061Hu01 | Polyclonal Antibody to Interleukin 15 (IL15) | WB; IHC; ICC; IP. |
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RAA061Hu21 | Recombinant Antibody to Interleukin 15 (IL15) | WB; IF; ICC; IHC; IP; FCM. |
MAA061Hu21 | Monoclonal Antibody to Interleukin 15 (IL15) | WB |
MAA061Hu22 | Monoclonal Antibody to Interleukin 15 (IL15) | WB; IHC; ICC; IP. |
SEA061Hu | ELISA Kit for Interleukin 15 (IL15) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA061Hu | Multiplex Assay Kit for Interleukin 15 (IL15) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
IGA061Hu21 | Mouse Knockin Human Interleukin 15 (IL15) | Animal Experiments; Positive Control. |