Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)

CSF2; GMCSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor

ACTIVITY TEST

TF-1, the human erythroleukemia cell line,provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. As reported, GM-CSF was also able to induce differentiation of human monoblastic leukemia cell line U937. In house data was obtained by the following experiment: TF-1 cells and U937 cells were incubated in the presence of various concentrations of rhGM-CSF, then cells were observed by inverted microscope everyday .
Cell proliferation of TF1 cells after incubation with GM-CSF (10ng/mL) for 3 days was shown in Figure 1.

Cell differentiation of U937 cells after incubation with GM-CSF (10ng/mL) for 5 days was shown in Figure 2.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Toxicology and Environmental Health Effects of 900-MHz Microwave Radiation on γ-Ray-Induced Damage to Mouse Hematopoietic System PubMed: 20391130
5 Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4×: a phase 2 randomised, double-blind, placebo-controlled trial Pubmed:24525316
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPA045Hu61 Eukaryotic Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Positive Control; Immunogen; SDS-PAGE; WB.
APA045Hu61 Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Cell culture; Activity Assays.
APA045Hu01 Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Cell culture; Activity Assays.
RPA045Hu01 Recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA045Hu01 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) WB; IHC; ICC; IP.
PAA045Hu06 Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) WB; IHC; ICC; IP.
MAA045Hu22 Monoclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) WB; IHC; ICC; IP.
SEA045Hu ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA045Hu CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Chemiluminescent immunoassay for Antigen Detection.
KSA045Hu01 ELISA Kit DIY Materials for Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Main materials for "Do It (ELISA Kit) Yourself".