≡

Active Amphiregulin (AREG)

AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor

ACTIVITY TEST

Amphiregulin (AREG) is a well-characterized member of the epidermal growth factor (EGF) family and is one of the ligands of the EGF receptor (EGFR). AREG plays a key role in mammalian development and in the control of branching morphogenesis in various organs. Furthermore, AREG participates in a wide range of physiological and pathological processes activating the major intracellular signalling cascades governing cell survival, proliferation and motility. Besides, EGF has been identified as an interactor of AREG, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human AREG and recombinant rat EGF. Briefly, AREG was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to EGF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AREG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human AREG and recombinant rat EGF was shown in Figure 1, the EC50 for this effect is 0.1 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Onkologie Amphiregulin and Epiregulin Expression in Colorectal Carcinoma and the Correlation with Clinicopathological Characteristics Karger: 315380
Br J Cancer. Serum levels of hepatocyte growth factor and epiregulin are associated with the prognosis on anti-EGFR antibody treatment in KRAS wild-type metastatic colorectal cancer. Pubmed:24800946
Cancer Sci Association between serum ligands and the skin toxicity of anti-epidermal growth factor receptor antibody in metastatic colorectal cancer PubMed: 25707609
J Cancer Res Clin Oncol Amphiregulin confers trastuzumab resistance via AKT and ERK activation in HER2-positive breast cancer PubMed: 26195282
Oncotarget.  Serum level of hepatocyte growth factor is a novel marker of predicting theoutcome and resistance to the treatment with trastuzumab in HER2-positive patients with metastatic gastric cancer Pubmed:26716644
Acta Pharmacologica Sinica Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi') Pubmed: 19363516
International Journal of Biological Sciences CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17
Neoplasia Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine Pubmed: 33212364
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets Pubmed: 33164313
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA006Hu01 Active Amphiregulin (AREG) Cell culture; Activity Assays.
RPA006Hu01 Recombinant Amphiregulin (AREG) Positive Control; Immunogen; SDS-PAGE; WB.
PAA006Hu01 Polyclonal Antibody to Amphiregulin (AREG) WB; IHC; ICC; IP.
MAA006Hu23 Monoclonal Antibody to Amphiregulin (AREG) WB; IHC; ICC; IP.
MAA006Hu25 Monoclonal Antibody to Amphiregulin (AREG) WB; IHC; ICC; IP.
MAA006Hu22 Monoclonal Antibody to Amphiregulin (AREG) WB,IHC
MAA006Hu24 Monoclonal Antibody to Amphiregulin (AREG) WB,IHC,ICC/IF
MAA006Hu26 Monoclonal Antibody to Amphiregulin (AREG) WB,IHC
MAA006Hu21 Monoclonal Antibody to Amphiregulin (AREG) WB; IHC; ICC; IP.
MAA006Hu27 Monoclonal Antibody to Amphiregulin (AREG) WB; IHC; ICC; IP.
MAA006Hu29 Monoclonal Antibody to Amphiregulin (AREG) WB; IHC; ICC; IP.
LAA006Hu76 Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) WB; IHC; ICC.
SEA006Hu ELISA Kit for Amphiregulin (AREG) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA006Hu CLIA Kit for Amphiregulin (AREG) Chemiluminescent immunoassay for Antigen Detection.
LMA006Hu Multiplex Assay Kit for Amphiregulin (AREG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA006Hu01 Antibody Pair for Amphiregulin (AREG) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA006Hu01 ELISA Kit DIY Materials for Amphiregulin (AREG) Main materials for "Do It (ELISA Kit) Yourself".