Active Angiotensin I Converting Enzyme (ACE)
CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form
- Product No.APA004Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point10.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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Figure. The binding activity of ACE with ACTb.
Angiotensin-converting enzyme ACE, is a central component of the renin-angiotensin system (RAS), which controls blood pressure by regulating the volume of fluids in the body. It converts the hormone angiotensin I to the active vasoconstrictor angiotensin II. Therefore, ACE indirectly increases blood pressure by causing blood vessels to constrict. ACE inhibitors are widely used as pharmaceutical drugs for treatment of cardiovascular diseases. Besides, Actin Beta (ACTb) has been identified as an interactor of ACE, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ACE and recombinant human ACTb. Briefly, ACE were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ACE pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ACE and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Lipids in Health and disease||Association of elevated blood pressure and impaired vasorelaxation in experimental Sprague-Dawley rats fed with heated vegetable oil PubMed: PMC2914008|
|Malaysian Journal of Medical Sciences||Effect of Repeatedly Heated Palm Olein on Blood Pressure–Regulating Enzymes Activity and Lipid Peroxidation in Rats Usm: MJMS19120|
|Journal of Cancer Therapy||Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. Scirp: Source|
|The American journal of clinical nutrition||Vascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial. Pubmed: 23553151|
|Journal of Vascular Surgery||Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice Pubmed:25037606|
|Reprod Toxicol.?||ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats Pubmed:25205467|
|Chemical Research in Toxicology||Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880|
|Chem Biol Interact||Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling PubMed: 26079204|
|Biomed Res Int||Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative Stress PubMed: 26640794|
|Clinics (Sao Paulo)||Mechanisms PubMed: 26602523|
|Chemical Research in Toxicology||Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880|
|Intensive Care Medicine Experimental||Angiotensin converting enzymes in patients with acute respiratory distress syndrome Content: 3|
|Evid Based Complement Alternat Med.||Garlic Attenuates Plasma and Kidney ACE-1 and AngII Modulations in Early Streptozotocin-Induced Diabetic Rats: Renal Clearance and Blood Pressure Implications Pubmed:27293465|
|Вавиловский журнал генетики и селекции||Ренин-ангиотензин-альдостероновая система у крыс линии НИСАГ (ISIAH) со стресс-индуцированной артериальной гипертензией 30_Dubinina.pdf|
|Animal Biology||The effect of heat stress on bull sperm quality and related HSPs expression content:journals|
|biomedicine & pharmacotherapy||Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. pubmed:28068636|
|Biomedicine & Pharmacotherapy||Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro- L -arginine methyl ester pubmed:28992471|
|Journal of Functional Foods||Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation 10.1016/j.jff.2017.05.033|
|Molecules||Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet Pubmed:29614743|
|Journal of Surgical Research||Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy Pubmed:29506841|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA004Hu01||Recombinant Angiotensin I Converting Enzyme (ACE)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA004Hu02||Recombinant Angiotensin I Converting Enzyme (ACE)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA004Hu02||Active Angiotensin I Converting Enzyme (ACE)||Cell culture; Activity Assays.|
|PAA004Hu01||Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE)||WB; ICC; IHC-P; IHC-F; ELISA.|
|PAA004Hu02||Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE)||WB; ICC; IHC-P; IHC-F; ELISA.|
|LAA004Hu81||FITC-Linked Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE)||WB; IHC; ICC; IF.|
|LAA004Hu71||Biotin-Linked Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE)||WB; IHC; ICC.|
|MAA004Hu22||Monoclonal Antibody to Angiotensin I Converting Enzyme (ACE)||WB; IHC; ICC; IP.|
|SEA004Hu||ELISA Kit for Angiotensin I Converting Enzyme (ACE)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA004Hu||CLIA Kit for Angiotensin I Converting Enzyme (ACE)||Chemiluminescent immunoassay for Antigen Detection.|