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Quality Guarantee
Certificate
| Product: | Anti-Beta-2-Microglobulin (b2M) Polyclonal Antibody |
| Target molecule: | Beta-2-Microglobulin (b2M) |
| Host: | Rabbit |
| Source: | Serum |
| Purification: | Affinity Chromatography |
| Isotype: | IgG |
| Traits: | Liquid |
| Concentration: | 200µg/mL |
| Buffer: | 0.01M PBS, pH7.4, containing 0.05% proclin-300, 50% glycerol. |
| Pairing: | RSA260Hu01 (capture antibody) is recommended to pair with RSA260Hu02 (detection antibody) in immunoassay. |
| Applications: | Recommended use: Recommended to be used in immunoassays for recognition of human b2M. Tested application: Capture antibody (1-4ug/mL) in ELISA assay. Detection antibody (0.5-10ug/mL) in WB. Detection antibody (5-40ug/mL) in IHC. |
| Storage: | Aliquot and store at -20°C for 12 months. Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. |
Identification of Specificity:
Figure 1. ELISA
Purified antigen and natural samples were tested in ELISA with pairing antibodies of RSA260Hu01 and RSA260Hu02.
Figure 2. WB
| Sample: | A. Recombinant Human b2M | |
| B. Lane 1: Human Leukocyte Cells | Lane 2: Human Serum | |
| Lane 3: Human Liver Tissue | Lane 4: Human Uterus |
Figure 3. IHC
| Sample: | A. Human Kidney Tissue | B. Human Kidney Cancer Tissue |
| C. Human Liver Tissue | D. Human Liver Cancer Tissue | |
| E. Human Testis Cancer Tissue | F. Human Stomach Tissue | |
| G. Human Rectum Tissue | H. Human Rectum Cancer Tissue | |
| I. Human Esophagus Cancer Tissue |