Product: Anti-Insulin Like Growth Factor 1 (IGF1) Monoclonal Antibody
Target molecule: Insulin Like Growth Factor 1 (IGF1)
Host: Mouse
Source: Ascites
Purification: Affinity Chromatography
Isotype: IgG2b, Kappa
Traits: Liquid
Concentration: 500µg/mL
Buffer: 0.01M PBS, pH7.4, containing 0.05% proclin-300, 50% glycerol.
Pairing: RSA050Hu21 (capture antibody) is recommended to pair with RSA050Hu22 (detection antibody) in immunoassay.
Applications: Recommended use: Recommended to be used in immunoassays for recognition of human IGF1. Tested application: Capture antibody (1-4ug/mL) in ELISA assay. Detection antibody (0.5-10ug/mL) in WB. Detection antibody (5-40ug/mL) in IHC.
Storage: Aliquot and store at -20°C for 12 months. Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use.

Identification of Specificity:

Figure 1. ELISA

Purified antigen and natural samples were tested in ELISA with paring antibodies of RSA050Hu21 and RSA050Hu22.

Figure 2. WB

Sample: A. Recombinant Human IGF1
B. Lane 1: Mouse Testis Tissue Lane2: Mouse Uterus Tissue

(Human IGF1 has several isforms by alternative splicing.)

Figure 3. IHC

Sample: Human Liver Tissue