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Catechol-O-Methyltransferase (COMT)

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Catechol-O-Methyltransferase (COMT)
CCC J365
HGNC 2228
MGI 88470
PubMed 22528689
RGD 2379
UniProt P21964
Wiki COMT
COMT is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines.
Catechol-O-methyl transferase is involved in the inactivation of the catecholamine neurotransmitters (dopamine, epinephrine, and norepinephrine). The enzyme introduces a methyl group to the catecholamine, which is donated by S-adenosyl methionine (SAM). COMT is an intracellular enzyme located in the postsynaptic neuron. Any compound having a catechol structure, like catecholestrogens and catechol-containing flavonoids, are substrates of COMT.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ365Hu01 Recombinant Catechol-O-Methyltransferase (COMT) Positive Control; Immunogen; SDS-PAGE; WB.
APJ365Hu01 Active Catechol-O-Methyltransferase (COMT) Cell culture; Activity Assays.
RPJ365Hu02 Recombinant Catechol-O-Methyltransferase (COMT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAJ365Hu01 Polyclonal Antibody to Catechol-O-Methyltransferase (COMT) WB,IHC,ICC/IF
MAJ365Hu21 Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) WB; IHC; ICC; IP.
MAJ365Hu22 Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) WB; IHC; ICC; IP.
Assay Kits SEJ365Hu ELISA Kit for Catechol-O-Methyltransferase (COMT) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ365Mu01 Recombinant Catechol-O-Methyltransferase (COMT) Positive Control; Immunogen; SDS-PAGE; WB.
RPJ365Mu02 Recombinant Catechol-O-Methyltransferase (COMT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAJ365Mu01 Polyclonal Antibody to Catechol-O-Methyltransferase (COMT) WB; IHC; ICC; IP.
PAJ365Mu02 Polyclonal Antibody to Catechol-O-Methyltransferase (COMT) WB; IHC; ICC; IP.
Assay Kits SEJ365Mu ELISA Kit for Catechol-O-Methyltransferase (COMT) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ365Ra01 Recombinant Catechol-O-Methyltransferase (COMT) Positive Control; Immunogen; SDS-PAGE; WB.
RPJ365Ra02 Recombinant Catechol-O-Methyltransferase (COMT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAJ365Ra01 Polyclonal Antibody to Catechol-O-Methyltransferase (COMT) WB; IHC; ICC; IP.
MAJ365Ra21 Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) WB; IHC
MAJ365Ra22 Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) WB; IHC; ICC; IP.
MAJ365Ra23 Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) WB; IHC; ICC; IP.
MAJ365Ra24 Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) WB; IHC; ICC; IP.
MAJ365Ra25 Monoclonal Antibody to Catechol-O-Methyltransferase (COMT) WB; IHC; ICC; IP.
Assay Kits SEJ365Ra ELISA Kit for Catechol-O-Methyltransferase (COMT) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Cloning and characterization of human placental catechol-O-methyltransferase cDNA."DNA Cell Biol. 10:181-189(1991) [PubMed] [Europe PMC] [Abstract]
  2. "Human catechol-O-methyltransferase: cloning and expression of the membrane-associated form."Proc. Natl. Acad. Sci. U.S.A. 88:1416-1420(1991) [PubMed] [Europe PMC] [Abstract]
  3. "Genomic organization of the human catechol O-methyltransferase gene and its expression from two distinct promoters."Eur. J. Biochem. 223:1049-1059(1994) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "A genome annotation-driven approach to cloning the human ORFeome."Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The DNA sequence of human chromosome 22." Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Mass spectrometric analysis of human soluble catechol O-methyltransferase expressed in Escherichia coli. Identification of a product of ribosomal frameshifting and of reactive cysteines involved in S-adenosyl-L-methionine binding."Eur. J. Biochem. 222:377-386(1994) [PubMed] [Europe PMC] [Abstract]
  9. "Purification and partial sequence analysis of the soluble catechol-O-methyltransferase from human placenta: comparison to the rat liver enzyme."Biochem. Biophys. Res. Commun. 174:995-1002(1991) [PubMed] [Europe PMC] [Abstract]
  10. "Cell-free synthesis of rat and human catechol O-methyltransferase. Insertion of the membrane-bound form into microsomal membranes in vitro."Eur. J. Biochem. 202:1013-1020(1991) [PubMed] [Europe PMC] [Abstract]
  11. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  12. "Orientation and cellular distribution of membrane-bound catechol-O-methyltransferase in cortical neurons: implications for drug development."J. Biol. Chem. 286:34752-34760(2011) [PubMed] [Europe PMC] [Abstract]
  13. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  14. "Crystal structures of human 108V and 108M catechol O-methyltransferase."J. Mol. Biol. 380:120-130(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Human catechol-O-methyltransferase pharmacogenetics: description of a functional polymorphism and its potential application to neuropsychiatric disorders."Pharmacogenetics 6:243-250(1996) [PubMed] [Europe PMC] [Abstract]
  16. "Association between the functional variant of the catechol-O-methyltransferase (COMT) gene and type 1 alcoholism."Mol. Psychiatry 4:286-289(1999) [PubMed] [Europe PMC] [Abstract]
  17. "Characterization of single-nucleotide polymorphisms in coding regions of human genes."Nat. Genet. 22:231-238(1999) [PubMed] [Europe PMC] [Abstract]
  18. ErratumNat. Genet. 23:373-373(1999)
  19. "Association of Ala72Ser polymorphism with COMT enzyme activity and the risk of schizophrenia in Koreans."Hum. Genet. 116:319-328(2005) [PubMed] [Europe PMC] [Abstract]
  20. "The V108M mutation decreases the structural stability of catechol O-methyltransferase."Biochim. Biophys. Acta 1784:1098-1105(2008) [PubMed] [Europe PMC] [Abstract]
  21. "Catechol O-methyltransferase val158-met polymorphism is associated with abdominal obesity and blood pressure in men."Metabolism 57:708-711(2008) [PubMed] [Europe PMC] [Abstract]