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Flap Structure Specific Endonuclease 1 (FEN1)

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MF1; RAD2; Maturation Factor-1; DNase IV

Flap Structure Specific Endonuclease 1 (FEN1)
CCC C491
HGNC 3650
MGI 102779
PubMed 19596913
RGD 621821
UniProt P39748
Wiki FEN1

Flap endonuclease 1 is an enzyme removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC491Hu01 Recombinant Flap Structure Specific Endonuclease 1 (FEN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Flap Structure Specific Endonuclease 1 (FEN1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Flap Structure Specific Endonuclease 1 (FEN1) Polyclonal Antibody Customized Service Offer
Assay Kits SEC491Hu ELISA Kit for Flap Structure Specific Endonuclease 1 (FEN1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Flap Structure Specific Endonuclease 1 (FEN1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Flap Structure Specific Endonuclease 1 (FEN1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Flap Structure Specific Endonuclease 1 (FEN1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Flap Structure Specific Endonuclease 1 (FEN1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Flap Structure Specific Endonuclease 1 (FEN1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Flap Structure Specific Endonuclease 1 (FEN1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Flap Structure Specific Endonuclease 1 (FEN1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Flap Structure Specific Endonuclease 1 (FEN1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Flap Structure Specific Endonuclease 1 (FEN1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Flap Structure Specific Endonuclease 1 (FEN1) ELISA Kit Customized Service Offer
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  7. "The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21."J. Biol. Chem. 272:24522-24529(1997) [PubMed] [Europe PMC] [Abstract]
  8. "Mechanism whereby proliferating cell nuclear antigen stimulates flap endonuclease 1."J. Biol. Chem. 275:10498-10505(2000) [PubMed] [Europe PMC] [Abstract]
  9. "Regulation of human flap endonuclease-1 activity by acetylation through the transcriptional coactivator p300."Mol. Cell 7:1221-1231(2001) [PubMed] [Europe PMC] [Abstract]
  10. "Arginine residues 47 and 70 of human flap endonuclease-1 are involved in DNA substrate interactions and cleavage site determination."J. Biol. Chem. 277:24659-24666(2002) [PubMed] [Europe PMC] [Abstract]
  11. "Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1."J. Biol. Chem. 283:20925-20936(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair."Mol. Cell. Biol. 28:4310-4319(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding."Nat. Chem. Biol. 6:766-773(2010) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  16. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  17. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  18. "A cryptic targeting signal creates a mitochondrial FEN1 isoform with tailed R-loop binding properties."PLoS ONE 8:E62340-E62340(2013) [PubMed] [Europe PMC] [Abstract]
  19. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  20. "Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-delta p66 subunit and flap endonuclease-1."Structure 12:2209-2219(2004) [PubMed] [Europe PMC] [Abstract]
  21. "Structural basis for recruitment of human flap endonuclease 1 to PCNA."EMBO J. 24:683-693(2005) [PubMed] [Europe PMC] [Abstract]