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Ki-67 Protein (Ki-67)

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MKI67; KIA; Ki67P; Antigen Identified By Monoclonal Antibody Ki-67

Ki-67 Protein (Ki-67)
CCC C047
HGNC 7107
MGI 106035
PubMed 20235278
RGD 1305476
UniProt P46013
Wiki Ki-67

The Ki-67 protein is a cellular marker for proliferation. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes.

Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).Ki-67 is an excellent marker to determine the growth fraction of a given cell population.

The fraction of Ki-67-positive tumor cells (the Ki-67 labelling index) is often correlated with the clinical course of cancer. The best-studied examples in this context are carcinomas of the prostate and the breast.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC047Hu01 Recombinant Ki-67 Protein (Ki-67) Positive Control; Immunogen; SDS-PAGE; WB.
RPC047Hu02 Recombinant Ki-67 Protein (Ki-67) Positive Control; Immunogen; SDS-PAGE; WB.
RPC047Hu03 Recombinant Ki-67 Protein (Ki-67) Positive Control; Immunogen; SDS-PAGE; WB.
EPC047Hu61 Eukaryotic Ki-67 Protein (Ki-67) Positive Control; Immunogen; SDS-PAGE; WB.
APC047Hu61 Active Ki-67 Protein (Ki-67) Cell culture; Activity Assays.
Antibodies PAC047Hu01 Polyclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
MAC047Hu22 Monoclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
PAC047Hu02 Polyclonal Antibody to Ki-67 Protein (Ki-67) WB; ICC; IHC-P; IHC-F; ELISA.
LAC047Hu71 Biotin-Linked Polyclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC.
LAC047Hu81 FITC-Linked Polyclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IF.
MAC047Hu23 Monoclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
MAC047Hu21 Monoclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
MAC047Hu24 Monoclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
Assay Kits SEC047Hu ELISA Kit for Ki-67 Protein (Ki-67) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC047Mu01 Recombinant Ki-67 Protein (Ki-67) Positive Control; Immunogen; SDS-PAGE; WB.
RPC047Mu02 Recombinant Ki-67 Protein (Ki-67) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC047Mu01 Polyclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
PAC047Mu02 Polyclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
Assay Kits SEC047Mu ELISA Kit for Ki-67 Protein (Ki-67) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC047Ra01 Recombinant Ki-67 Protein (Ki-67) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC047Ra01 Polyclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
MAC047Ra21 Monoclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
Assay Kits SEC047Ra ELISA Kit for Ki-67 Protein (Ki-67) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC047Rb01 Recombinant Ki-67 Protein (Ki-67) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC047Rb01 Polyclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Ki-67 Protein (Ki-67) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ki-67 Protein (Ki-67) ELISA Kit Customized Service Offer
  1. "The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins."J. Cell Biol. 123:513-522(1993) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The forkhead-associated domain of Ki-67 antigen interacts with the novel kinesin-like protein Hklp2."J. Biol. Chem. 275:28888-28892(2000) [PubMed] [Europe PMC] [Abstract]
  4. "A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis."J. Biol. Chem. 276:25386-25391(2001) [PubMed] [Europe PMC] [Abstract]
  5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  6. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  7. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  8. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  10. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Identification and characterization of a novel nuclear protein complex involved in nuclear hormone receptor-mediated gene regulation."J. Biol. Chem. 284:7542-7552(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  18. "Microcephaly gene links trithorax and REST/NRSF to control neural stem cell proliferation and differentiation."Cell 151:1097-1112(2012) [PubMed] [Europe PMC] [Abstract]
  19. "Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization."Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract]
  20. "Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions."J. Mol. Biol. 335:371-381(2004) [PubMed] [Europe PMC] [Abstract]
  21. "Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67."Nat. Struct. Mol. Biol. 12:987-993(2005) [PubMed] [Europe PMC] [Abstract]