≡

Coagulation Factor X (F10)

[Edit]

FX; FXA; Stuart Prower Factor; Thrombokinase

Coagulation Factor X (F10)
CCC A834
HGNC 3528
MGI 103107
PubMed 11867437
RGD 61850
UniProt P00742
Wiki FX

As the only known physiological activator of thrombin, factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human factor X is initially synthesized in the liver as a single-chain precursor of 488 amino acid residues with a signal peptide and a pro region (residues 1 - 40). Both the intrinsic and extrinsic pathways activate factor X to Xa, which consists of light (residues 41 - 179) and heavy (residues 235 - 488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain. The full-length human factor X was expressed and the pro enzyme was purified and activated. The active protease was further purified and analyzed for its activity towards either peptides or proteins containing a Xa cleavage site.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA834Hu01 Recombinant Coagulation Factor X (F10) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA834Hu01 Polyclonal Antibody to Coagulation Factor X (F10) WB; IHC; ICC; IP.
MAA834Hu22 Monoclonal Antibody to Coagulation Factor X (F10) WB; IHC; ICC; IP.
Assay Kits SEA834Hu ELISA Kit for Coagulation Factor X (F10) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA834Hu CLIA Kit for Coagulation Factor X (F10) Chemiluminescent immunoassay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA834Mu01 Recombinant Coagulation Factor X (F10) Positive Control; Immunogen; SDS-PAGE; WB.
RPA834Mu02 Recombinant Coagulation Factor X (F10) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA834Mu01 Polyclonal Antibody to Coagulation Factor X (F10) WB; IHC; ICC; IP.
PAA834Mu02 Polyclonal Antibody to Coagulation Factor X (F10) WB; IHC; ICC; IP.
Assay Kits SEA834Mu ELISA Kit for Coagulation Factor X (F10) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA834Ra01 Recombinant Coagulation Factor X (F10) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA834Ra01 Polyclonal Antibody to Coagulation Factor X (F10) WB; IHC; ICC; IP.
MAA834Ra21 Monoclonal Antibody to Coagulation Factor X (F10) WB; IHC; ICC; IP.
Assay Kits SEA834Ra ELISA Kit for Coagulation Factor X (F10) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Cloning and expression in COS-1 cells of a full-length cDNA encoding human coagulation factor X."Gene 99:291-294(1991) [PubMed] [Europe PMC] [Abstract]
  2. "Gene for human factor X: a blood coagulation factor whose gene organization is essentially identical with that of factor IX and protein C."Biochemistry 25:5098-5102(1986) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Characterization of an almost full-length cDNA coding for human blood coagulation factor X."Proc. Natl. Acad. Sci. U.S.A. 82:3591-3595(1985) [PubMed] [Europe PMC] [Abstract]
  5. "Isolation and characterization of human blood-coagulation factor X cDNA."Gene 41:311-314(1986) [PubMed] [Europe PMC] [Abstract]
  6. "Complete amino acid sequence of the light chain of human blood coagulation factor X: evidence for identification of residue 63 as beta-hydroxyaspartic acid."Biochemistry 22:2875-2884(1983) [PubMed] [Europe PMC] [Abstract]
  7. "Characterization of a cDNA coding for human factor X."Proc. Natl. Acad. Sci. U.S.A. 81:3699-3702(1984) [PubMed] [Europe PMC] [Abstract]
  8. "Identification of O-linked oligosaccharide chains in the activation peptides of blood coagulation factor X. The role of the carbohydrate moieties in the activation of factor X."Eur. J. Biochem. 218:153-163(1993) [PubMed] [Europe PMC] [Abstract]
  9. "Cloning and characterization of the 5' end (exon 1) of the gene encoding human factor X."Gene 84:517-519(1989) [PubMed] [Europe PMC] [Abstract]
  10. "Mechanism of inhibition of activated protein C by protein C inhibitor."J. Biochem. 95:187-195(1984) [PubMed] [Europe PMC] [Abstract]
  11. "Basis for the specificity and activation of the serpin protein Z-dependent proteinase inhibitor (ZPI) as an inhibitor of membrane-associated factor Xa."J. Biol. Chem. 285:20399-20409(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
  13. "Structure of human des(1-45) factor Xa at 2.2-A resolution."J. Mol. Biol. 232:947-966(1993) [PubMed] [Europe PMC] [Abstract]
  14. "Structural basis for chemical inhibition of human blood coagulation factor Xa."Proc. Natl. Acad. Sci. U.S.A. 95:6630-6635(1998) [PubMed] [Europe PMC] [Abstract]
  15. "Substituted thiophene-anthranilamides as potent inhibitors of human factor Xa." Bioorg. Med. Chem. 15:2127-2146(2007) [PubMed] [Europe PMC] [Abstract]
  16. "Molecular characterization of human factor XSan Antonio."Blood 74:1486-1490(1989) [PubMed] [Europe PMC] [Abstract]
  17. "Molecular defect (Gla+14TO: hereditary factor X deficiency (factor X 'Vorarlberg')."J. Biol. Chem. 265:11982-11989(1990) [PubMed] [Europe PMC] [Abstract]
  18. "Molecular defect in coagulation factor XFriuli results from a substitution of serine for proline at position 343."Blood 77:317-323(1991) [PubMed] [Europe PMC] [Abstract]
  19. "Molecular bases of CRM+ factor X deficiency: a frequent mutation (Ser334Pro) in the catalytic domain and a substitution (Glu102Lys) in the second EGF-like domain."Br. J. Haematol. 90:910-915(1995) [PubMed] [Europe PMC] [Abstract]
  20. "Functional consequences of the Ser334-->Pro mutation in a human factor X variant (factor XMarseille)."Eur. J. Biochem. 234:140-147(1995) [PubMed] [Europe PMC] [Abstract]
  21. "Factor XKetchikan: a variant molecule in which Gly replaces a Gla residue at position 14 in the light chain."Hum. Genet. 95:212-214(1995) [PubMed] [Europe PMC] [Abstract]
  22. "Factor X Stockton: a mild bleeding diathesis associated with an active site mutation in factor X."Blood Coagul. Fibrinolysis 7:5-14(1996) [PubMed] [Europe PMC] [Abstract]
  23. "Factor XSt. Louis II. Identification of a glycine substitution at residue 7 and characterization of the recombinant protein."J. Biol. Chem. 271:28601-28606(1996) [PubMed] [Europe PMC] [Abstract]
  24. "A family with hereditary factor X deficiency with a point mutation Gla32 to Gln in the Gla domain (factor X Tokyo)."Br. J. Haematol. 106:809-811(1999) [PubMed] [Europe PMC] [Abstract]
  25. "Characterization of single-nucleotide polymorphisms in coding regions of human genes."Nat. Genet. 22:231-238(1999) [PubMed] [Europe PMC] [Abstract]
  26. ErratumNat. Genet. 23:373-373(1999)
  27. "Molecular analysis of the genotype-phenotype relationship in factor X deficiency."Hum. Genet. 106:249-257(2000) [PubMed] [Europe PMC] [Abstract]
  28. "The impact of Glu102Lys on the factor X function in a patient with a doubly homozygous factor X deficiency (Gla14Lys and Glu102Lys)."Thromb. Haemost. 83:234-238(2000) [PubMed] [Europe PMC] [Abstract]
  29. "A dysfunctional factor X (factor X San Giovanni Rotondo) present at homozygous and double heterozygous level: identification of a novel microdeletion (delC556) and missense mutation (Lys(408)-->Asn) in the factor X gene. A study of an Italian family."Thromb. Res. 101:219-230(2001) [PubMed] [Europe PMC] [Abstract]
  30. "A new factor X defect (factor X Padua 3): a compound heterozygous between true deficiency (Gly(380)-->Arg) and an abnormality (Ser(334)-->Pro)."Thromb. Res. 104:257-264(2001) [PubMed] [Europe PMC] [Abstract]
  31. "A novel type I factor X variant (factor X Cys350Phe) due to loss of a disulfide bond in the catalytic domain."Blood Coagul. Fibrinolysis 14:401-405(2003) [PubMed] [Europe PMC] [Abstract]
  32. "Impaired prothrombinase activity of factor X Gly381Asp results in severe familial CRM+ FX deficiency."Thromb. Haemost. 89:243-248(2003) [PubMed] [Europe PMC] [Abstract]
  33. "Molecular characterization of factor X deficiency associated with borderline plasma factor X level."Haematologica 89:501-502(2004) [PubMed] [Europe PMC] [Abstract]
  34. "Genetic analysis of hereditary factor X deficiency in a French patient of Sri Lankan ancestry: in vitro expression study identified Gly366Ser substitution as the molecular basis of the dysfunctional factor X."Blood Coagul. Fibrinolysis 16:9-16(2005) [PubMed] [Europe PMC] [Abstract]
  35. "Analysis of the novel factor X gene mutation Glu51Lys in two families with factor X-Riyadh anomaly."Thromb. Haemost. 97:542-545(2007) [PubMed] [Europe PMC] [Abstract]
  36. "Characterization of a homozygous Gly11Val mutation in the Gla domain of coagulation factor X."Thromb. Res. 124:144-148(2009) [PubMed] [Europe PMC] [Abstract]
  37. "Molecular dynamics characterization of five pathogenic Factor X mutants associated with decreased catalytic activity."Biochemistry 53:6992-7001(2014) [PubMed] [Europe PMC] [Abstract]