Beta-Site APP Cleaving Enzyme 1 (bACE1)

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ASP2; Beta Secretase; Memapsin 2; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1; Membrane-associated aspartic protease 2

Beta-Site APP Cleaving Enzyme 1 (bACE1)
β-Secretaseis an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein, contains two active site aspartate residues in its extracellular protein domain and may function as a dimer.Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the amyloid precursor protein (APP). Extracellular cleavage of APP by BACE releases a soluble extracellular fragment and is followed by APP cleavage within its transmembrane domain by γ-secretase. The second cleavage releases the intracellular domain of APP and amyloid-β. Since alpha-secretase cleaves APP closer to the cell membrane than BACE does.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA718Hu01 Recombinant Beta-Site APP Cleaving Enzyme 1 (bACE1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA718Hu01 Polyclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) WB; IHC; ICC; IP.
MAA718Hu22 Monoclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) WB
MAA718Hu21 Monoclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) WB; IHC; ICC; IP.
MAA718Hu23 Monoclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) WB; IHC; ICC; IP.
MAA718Hu24 Monoclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) WB; IHC; ICC; IP.
MAA718Hu25 Monoclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) WB; IHC; ICC; IP.
Assay Kits SEA718Hu ELISA Kit for Beta-Site APP Cleaving Enzyme 1 (bACE1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA718Mu01 Recombinant Beta-Site APP Cleaving Enzyme 1 (bACE1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA718Mu02 Recombinant Beta-Site APP Cleaving Enzyme 1 (bACE1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA718Mu01 Polyclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) WB; IHC; ICC; IP.
PAA718Mu02 Polyclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) WB; IHC; ICC; IP.
Assay Kits SEA718Mu ELISA Kit for Beta-Site APP Cleaving Enzyme 1 (bACE1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA718Ra01 Recombinant Beta-Site APP Cleaving Enzyme 1 (bACE1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA718Ra01 Polyclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) WB; IHC; ICC; IP.
MAA718Ra21 Monoclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) WB; IHC; ICC; IP.
Assay Kits SEA718Ra ELISA Kit for Beta-Site APP Cleaving Enzyme 1 (bACE1) Enzyme-linked immunosorbent assay for Antigen Detection.
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  5. "Three novel alternatively spliced isoforms of the human beta-site amyloid precursor protein cleaving enzyme (BACE) and their effect on amyloid beta-peptide production."Neurosci. Lett. 307:9-12(2001) [PubMed] [Europe PMC] [Abstract]
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  11. "ASP1 (BACE2) cleaves the amyloid precursor protein at the beta-secretase site."Mol. Cell. Neurosci. 16:609-619(2000) [PubMed] [Europe PMC] [Abstract]
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  13. "The disulphide bonds in the catalytic domain of BACE are critical but not essential for amyloid precursor protein processing activity."J. Neurochem. 80:1079-1088(2002) [PubMed] [Europe PMC] [Abstract]
  14. "Biochemical and structural characterization of the interaction of memapsin 2 (beta-secretase) cytosolic domain with the VHS domain of GGA proteins."Biochemistry 42:12174-12180(2003) [PubMed] [Europe PMC] [Abstract]
  15. "Reticulon family members modulate BACE1 activity and amyloid-beta peptide generation."Nat. Med. 10:959-965(2004) [PubMed] [Europe PMC] [Abstract]
  16. "Reticulons RTN3 and RTN4-B/C interact with BACE1 and inhibit its ability to produce amyloid beta-protein."Eur. J. Neurosci. 24:1237-1244(2006) [PubMed] [Europe PMC] [Abstract]
  17. "Mapping of interaction domains mediating binding between BACE1 and RTN/Nogo proteins."J. Mol. Biol. 363:625-634(2006) [PubMed] [Europe PMC] [Abstract]
  18. "A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1."Biochem. J. 407:383-395(2007) [PubMed] [Europe PMC] [Abstract]
  19. "PCSK9 is required for the disposal of non-acetylated intermediates of the nascent membrane protein BACE1."EMBO Rep. 9:916-922(2008) [PubMed] [Europe PMC] [Abstract]
  20. "Two endoplasmic reticulum (ER)/ER Golgi intermediate compartment-based lysine acetyltransferases post-translationally regulate BACE1 levels."J. Biol. Chem. 284:2482-2492(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Proteomic identification of sorting nexin 6 as a negative regulator of BACE1-mediated APP processing."FASEB J. 24:2783-2794(2010) [PubMed] [Europe PMC] [Abstract]
  22. "Structure of the protease domain of memapsin 2 (beta-secretase) complexed with inhibitor."Science 290:150-153(2000) [PubMed] [Europe PMC] [Abstract]
  23. "Crystal structure of memapsin 2 (beta-secretase) in complex with an inhibitor OM00-3."Biochemistry 41:10963-10967(2002) [PubMed] [Europe PMC] [Abstract]
  24. "Flap position of free memapsin 2 (beta-secretase), a model for flap opening in aspartic protease catalysis."Biochemistry 43:4689-4695(2004) [PubMed] [Europe PMC] [Abstract]
  25. "Apo and inhibitor complex structures of BACE (beta-secretase)."J. Mol. Biol. 343:407-416(2004) [PubMed] [Europe PMC] [Abstract]
  26. "Structural locations and functional roles of new subsites S5, S6, and S7 in memapsin 2 (beta-secretase)."Biochemistry 44:105-112(2005) [PubMed] [Europe PMC] [Abstract]