Caspase 3 (CASP3)

[Edit]

CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1

Caspase 3 (CASP3)

Caspase-3 (CPP-32, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA626Hu01 Recombinant Caspase 3 (CASP3) Positive Control; Immunogen; SDS-PAGE; WB.
RPA626Hu02 Recombinant Caspase 3 (CASP3) Positive Control; Immunogen; SDS-PAGE; WB.
RPA626Hu03 Recombinant Caspase 3 (CASP3) Positive Control; Immunogen; SDS-PAGE; WB.
APA626Hu01 Active Caspase 3 (CASP3) Cell culture; Activity Assays.
Antibodies PAA626Hu01 Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
MAA626Hu22 Monoclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
LAA626Hu71 Biotin-Linked Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC.
LAA626Hu81 FITC-Linked Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IF.
PAA626Hu02 Polyclonal Antibody to Caspase 3 (CASP3) WB,IHC
MAA626Hu21 Monoclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
PAA626Hu03 Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
Assay Kits SEA626Hu ELISA Kit for Caspase 3 (CASP3) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA626Mu01 Recombinant Caspase 3 (CASP3) Positive Control; Immunogen; SDS-PAGE; WB.
RPA626Mu02 Recombinant Caspase 3 (CASP3) Positive Control; Immunogen; SDS-PAGE; WB.
APA626Mu02 Active Caspase 3 (CASP3) Cell culture; Activity Assays.
Antibodies PAA626Mu01 Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
LAA626Mu71 Biotin-Linked Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC.
LAA626Mu81 FITC-Linked Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IF.
PAA626Mu02 Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
Assay Kits SEA626Mu ELISA Kit for Caspase 3 (CASP3) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA626Ra01 Recombinant Caspase 3 (CASP3) Positive Control; Immunogen; SDS-PAGE; WB.
RPA626Ra02 Recombinant Caspase 3 (CASP3) Positive Control; Immunogen; SDS-PAGE; WB.
APA626Ra03 Active Caspase 3 (CASP3) Cell culture; Activity Assays.
Antibodies PAA626Ra01 Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
PAA626Ra02 Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
LAA626Ra71 Biotin-Linked Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC.
LAA626Ra81 FITC-Linked Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IF.
MAA626Ra21 Monoclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
LAA626Ra82 FITC-Linked Monoclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IF.
PAA626Ra03 Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
PAA626Ra04 Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
Assay Kits SEA626Ra ELISA Kit for Caspase 3 (CASP3) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA626Ra High Sensitive ELISA Kit for Caspase 3 (CASP3) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Caspase 3 (CASP3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Caspase 3 (CASP3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Caspase 3 (CASP3) Polyclonal Antibody Customized Service Offer
Assay Kits SEA626Rb ELISA Kit for Caspase 3 (CASP3) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA626Bo01 Recombinant Caspase 3 (CASP3) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA626Bo01 Polyclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
MAA626Bo21 Monoclonal Antibody to Caspase 3 (CASP3) WB; IHC; ICC; IP.
Assay Kits SEA626Bo ELISA Kit for Caspase 3 (CASP3) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme."J. Biol. Chem. 269:30761-30764(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase."Cell 81:801-809(1995) [PubMed] [Europe PMC] [Abstract]
  3. "Caspase 3 activation is controlled by a sequence located in the N-terminus of its large subunit."Biochem. Biophys. Res. Commun. 316:93-99(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis."Nature 376:37-43(1995) [PubMed] [Europe PMC] [Abstract]
  7. "In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains."Proc. Natl. Acad. Sci. U.S.A. 93:7464-7469(1996) [PubMed] [Europe PMC] [Abstract]
  8. "Cleavage of huntingtin by apopain, a proapoptotic cysteine protease, is modulated by the polyglutamine tract."Nat. Genet. 13:442-449(1996) [PubMed] [Europe PMC] [Abstract]
  9. "Fas-induced caspase denitrosylation."Science 284:651-654(1999) [PubMed] [Europe PMC] [Abstract]
  10. "Dual role of BRUCE as an antiapoptotic IAP and a chimeric E2/E3 ubiquitin ligase."Mol. Cell 14:801-811(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  13. "RET modulates cell adhesion via its cleavage by caspase in sympathetic neurons."J. Biol. Chem. 286:14628-14638(2011) [PubMed] [Europe PMC] [Abstract]
  14. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  15. "The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis."Nat. Struct. Biol. 3:619-625(1996) [PubMed] [Europe PMC] [Abstract]
  16. "Structure of recombinant human CPP32 in complex with the tetrapeptide acetyl-Asp-Val-Ala-Asp fluoromethyl ketone."J. Biol. Chem. 272:6539-6547(1997) [PubMed] [Europe PMC] [Abstract]
  17. "Potent and selective nonpeptide inhibitors of caspases 3 and 7 inhibit apoptosis and maintain cell functionality." J. Biol. Chem. 275:16007-16014(2000) [PubMed] [Europe PMC] [Abstract]