Carboxypeptidase B2 (CPB2)

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TAFI; CPU; PCPB; Thrombin Activatable Fibrinolysis Inhibitor; Plasma Carboxypeptidase B; Carboxypeptidase U

Carboxypeptidase B2 (CPB2)

TAFI, Thrombin Activatable Fibrinolytic Inhibitor, (also known as carboxypeptidase U and plasma carboxypeptidase B) is a 60,000 D molecular ratio glycoprotein (proenzyme form) present in rabbit plasma1 that modulates fibrinolysis in vivo. This proenzyme is converted to a 35,000 D molecular ratio active form, TAFIa, following proteolytic cleavage by the thrombin/ thrombomodulin complex. TAFIa possesses carboxypeptidase activity with a preference for cleaving lysine and arginine residues from the c-terminus of proteins. Modulation of fibrinolysis occurs when TAFIa cleaves C-terminal arginine and lysine residues of partially degraded fibrin.2,4,5 The removal of the c-terminus arginine and lysine residues from fibrin inhibits the continued degradation of fibrin by tPA activated plasmin.3

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA615Hu01 Recombinant Carboxypeptidase B2 (CPB2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA615Hu01 Polyclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC; IP.
MAA615Hu22 Monoclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC; IP.
Assay Kits SEA615Hu ELISA Kit for Carboxypeptidase B2 (CPB2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA615Mu01 Recombinant Carboxypeptidase B2 (CPB2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA615Mu02 Recombinant Carboxypeptidase B2 (CPB2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA615Mu03 Recombinant Carboxypeptidase B2 (CPB2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA615Mu01 Polyclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC; IP.
PAA615Mu03 Polyclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC; IP.
PAA615Mu02 Polyclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC; IP.
LAA615Mu71 Biotin-Linked Polyclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC.
LAA615Mu81 FITC-Linked Polyclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC; IF.
Assay Kits SEA615Mu ELISA Kit for Carboxypeptidase B2 (CPB2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA615Ra01 Recombinant Carboxypeptidase B2 (CPB2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA615Ra01 Polyclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC; IP.
MAA615Ra21 Monoclonal Antibody to Carboxypeptidase B2 (CPB2) WB; IHC; ICC; IP.
Assay Kits SEA615Ra ELISA Kit for Carboxypeptidase B2 (CPB2) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Isolation, molecular cloning, and partial characterization of a novel carboxypeptidase B from human plasma."J. Biol. Chem. 266:21833-21838(1991) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and analysis of human chromosome 13." Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Characterization of plasmin-mediated activation of plasma procarboxypeptidase B. Modulation by glycosaminoglycans."J. Biol. Chem. 274:35046-35052(1999) [PubMed] [Europe PMC] [Abstract]
  6. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
  7. "Post-translational modifications of human thrombin-activatable fibrinolysis inhibitor (TAFI): evidence for a large shift in the isoelectric point and reduced solubility upon activation."Biochemistry 45:1525-1535(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  9. "Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
  10. "Crystal structures of TAFI elucidate the inactivation mechanism of activated TAFI: a novel mechanism for enzyme autoregulation."Blood 112:2803-2809(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Insights into the molecular inactivation mechanism of human activated thrombin-activatable fibrinolysis inhibitor."J. Thromb. Haemost. 8:1056-1065(2010) [PubMed] [Europe PMC] [Abstract]