Protein Kinase D2 (PKD2)

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PRKD2; PRK-D2; PRKD-2; PK-D2; HSPC187; nPKC-D2; Serine/threonine-protein kinase D2

Protein Kinase D2 (PKD2)

Serine/threonine-protein kinase D2 is  encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms.

The addition of phosphatidyl-L-serine caused synergistic stimulation. Phorbol ester-activated PRKD2 migrated more slowly than inactive PRKD2 by SDS-PAGE, suggesting hyperphosphorylation upon activation.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA510Hu01 Recombinant Protein Kinase D2 (PKD2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA510Hu01 Polyclonal Antibody to Protein Kinase D2 (PKD2) WB; ICC; IHC-P; IHC-F; ELISA.
Assay Kits n/a CLIA Kit for Protein Kinase D2 (PKD2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase D2 (PKD2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA510Mu01 Recombinant Protein Kinase D2 (PKD2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA510Mu01 Polyclonal Antibody to Protein Kinase D2 (PKD2) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Protein Kinase D2 (PKD2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase D2 (PKD2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase D2 (PKD2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase D2 (PKD2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase D2 (PKD2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase D2 (PKD2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase D2 (PKD2) ELISA Kit Customized Service Offer
  1. "Molecular cloning and characterization of the human protein kinase D2. A novel member of the protein kinase d family of serine threonine kinases."J. Biol. Chem. 276:3310-3318(2001) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  5. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
  6. "Mechanism of activation of protein kinase D2(PKD2) by the CCK(B)/gastrin receptor."J. Biol. Chem. 277:29431-29436(2002) [PubMed] [Europe PMC] [Abstract]
  7. "Protein kinase D2 mediates activation of nuclear factor kappaB by Bcr-Abl in Bcr-Abl+ human myeloid leukemia cells."Cancer Res. 64:8939-8944(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network."Nat. Cell Biol. 6:106-112(2004) [PubMed] [Europe PMC] [Abstract]
  9. "Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells."Int. Immunol. 18:1737-1747(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Protein kinase D2 mediates lysophosphatidic acid-induced interleukin 8 production in nontransformed human colonic epithelial cells through NF-kappaB."Am. J. Physiol. 292:C767-C777(2007) [PubMed] [Europe PMC] [Abstract]
  11. "Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2."EMBO J. 26:4619-4633(2007) [PubMed] [Europe PMC] [Abstract]
  12. "Angiotensin II directly triggers endothelial exocytosis via protein kinase C-dependent protein kinase D2 activation."J. Pharmacol. Sci. 105:168-176(2007) [PubMed] [Europe PMC] [Abstract]
  13. "Selective binding of phorbol esters and diacylglycerol by individual C1 domains of the PKD family."Biochem. J. 411:333-342(2008) [PubMed] [Europe PMC] [Abstract]
  14. "Protein kinase D2 regulates chromogranin A secretion in human BON neuroendocrine tumour cells."Cell. Signal. 20:925-934(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  16. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  18. "PKD2 interacts with Lck and regulates NFAT activity in T cells."BMB Rep. 42:35-40(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Identification of protein kinase D2 as a pivotal regulator of endothelial cell proliferation, migration, and angiogenesis."J. Biol. Chem. 284:799-806(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  23. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  24. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  25. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  26. "A novel splice variant of calcium and integrin-binding protein 1 mediates protein kinase D2-stimulated tumour growth by regulating angiogenesis."Oncogene 33:1167-1180(2014) [PubMed] [Europe PMC] [Abstract]
  27. "Solution structure of the PH domain of protein kinase C, D2 type from human."Submitted (NOV-2005) to the PDB data bank
  28. "Phosphorylation-dependent interaction between antigenic peptides and MHC class I: a molecular basis for the presentation of transformed self."Nat. Immunol. 9:1236-1243(2008) [PubMed] [Europe PMC] [Abstract]
  29. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]