Ribonuclease H (RNASEH)

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Rnase-H; RNASEH1; RNH1; Ribonuclease H type II

Ribonuclease H (RNASEH)

The enzyme RNase H is a ribonuclease that cleaves the 3'-O-P-bond of RNA in a DNA/RNA duplex to produce 3'-hydroxyl and 5'-phosphate terminated products. RNase H is a non-specific endonuclease and catalyzes the cleavage of RNA via an hydrolytic mechanism, aided by an enzyme-bound divalent metal ion.

Members of the RNase H family can be found in nearly all organisms, from archaea and prokaryota to eukaryota. In DNA replication, RNase H is responsible for cutting out the RNA primer, allowing completion of the newly synthesized DNA.

Retroviral RNase H, a part of the viral reverse transcriptase enzyme, is an important pharmaceutical target, as it is absolutely necessary for the proliferation of retroviruses, such as HIV.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA200Hu01 Recombinant Ribonuclease H (RNASEH) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA200Hu01 Polyclonal Antibody to Ribonuclease H (RNASEH) WB; IHC; ICC; IP.
MAA200Hu22 Monoclonal Antibody to Ribonuclease H (RNASEH) WB; IHC; ICC; IP.
Assay Kits SEA200Hu ELISA Kit for Ribonuclease H (RNASEH) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ribonuclease H (RNASEH) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ribonuclease H (RNASEH) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ribonuclease H (RNASEH) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ribonuclease H (RNASEH) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ribonuclease H (RNASEH) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ribonuclease H (RNASEH) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ribonuclease H (RNASEH) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ribonuclease H (RNASEH) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ribonuclease H (RNASEH) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ribonuclease H (RNASEH) ELISA Kit Customized Service Offer
  1. "Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI."Genomics 53:300-307(1998) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning and expression of cDNA for human RNase H."Antisense Nucleic Acid Drug Dev. 8:53-61(1998) [PubMed] [Europe PMC] [Abstract]
  3. "Cloning, subcellular localization and functional expression of human RNase HII."Biol. Chem. 379:1407-1412(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
  5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Properties of cloned and expressed human RNase H1."J. Biol. Chem. 274:28270-28278(1999) [PubMed] [Europe PMC] [Abstract]
  8. "Human senataxin resolves RNA/DNA hybrids formed at transcriptional pause sites to promote Xrn2-dependent termination."Mol. Cell 42:794-805(2011) [PubMed] [Europe PMC] [Abstract]
  9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]