TEK Tyrosine Kinase, Endothelial (Tie2)

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CD202b; CD202-B; TEK; VMCM1; Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 2; Venous Malformations Multiple Cutaneous And Mucosal

TEK Tyrosine Kinase, Endothelial (Tie2)

Tie-2 is a receptor tyrosine kinase that is expressed primarily on endothelial cells (ECs) and plays a critical role in vascular development. The Tie-2 gene encodes a protein of 1122 amino acids. The extracellular region has three distinct structural motifs including two immunoglobulin (Ig)-like loops separated by three EGF-like repeats, and three repeats with fibronectin type III homology located after the second Ig loop. The intracellular portion of Tie-2 contains two tyrosine kinase domains that, when phosphorylated, interact with a number of binding partners including Grb2, Grb7, Grb14, Shp2, the p85 subunit of phosphatidylinositol 3-kinase (PI3K), and Dok-R. Deletion of the last 16 amino acids of the intracellular C-terminus results in increased levels of autophosphorylation, suggesting that it may play an autoinhibitory role.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA126Hu01 Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA126Hu02 Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA126Hu01 Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) WB; IHC; ICC; IP.
MAA126Hu22 Monoclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) WB; IHC; ICC; IP.
PAA126Hu02 Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) WB; IHC; ICC; IP.
Assay Kits SEA126Hu ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA126Mu01 Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA126Mu02 Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA126Mu01 Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) WB; IHC; ICC; IP.
PAA126Mu02 Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) WB; IHC; ICC; IP.
Assay Kits SEA126Mu ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA126Ra01 Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA126Ra01 Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) WB; IHC; ICC; IP.
MAA126Ra21 Monoclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) WB; IHC; ICC; IP.
Assay Kits SEA126Ra ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) CLIA Kit Customized Service Offer
n/a ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit Customized Service Offer

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA126Bo01 Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA126Bo01 Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) CLIA Kit Customized Service Offer
n/a ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit Customized Service Offer

Organism species: Danio rerio (Zebrafish)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA126Fi01 Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA126Fi01 Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) CLIA Kit Customized Service Offer
n/a ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit Customized Service Offer
  1. "Molecular cloning and characterization of a novel receptor protein tyrosine kinase from human placenta."Oncogene 8:663-670(1993) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "DNA sequence and analysis of human chromosome 9." Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis."Science 277:55-60(1997) [PubMed] [Europe PMC] [Abstract]
  7. "Identification of a soluble form of the angiopoietin receptor TIE-2 released from endothelial cells and present in human blood."Angiogenesis 4:123-131(2001) [PubMed] [Europe PMC] [Abstract]
  8. "Mechanistic effects of autophosphorylation on receptor tyrosine kinase catalysis: enzymatic characterization of Tie2 and phospho-Tie2."Biochemistry 40:10243-10253(2001) [PubMed] [Europe PMC] [Abstract]
  9. "Tie-2-dependent activation of RhoA and Rac1 participates in endothelial cell motility triggered by angiopoietin-1."Blood 102:2482-2490(2003) [PubMed] [Europe PMC] [Abstract]
  10. "The antiinflammatory endothelial tyrosine kinase Tie2 interacts with a novel nuclear factor-kappaB inhibitor ABIN-2."Circ. Res. 92:630-636(2003) [PubMed] [Europe PMC] [Abstract]
  11. "Biological characterization of angiopoietin-3 and angiopoietin-4."FASEB J. 18:1200-1208(2004) [PubMed] [Europe PMC] [Abstract]
  12. "Adaptor ShcA protein binds tyrosine kinase Tie2 receptor and regulates migration and sprouting but not survival of endothelial cells."J. Biol. Chem. 279:13224-13233(2004) [PubMed] [Europe PMC] [Abstract]
  13. "Multiple angiopoietin recombinant proteins activate the Tie1 receptor tyrosine kinase and promote its interaction with Tie2."J. Cell Biol. 169:239-243(2005) [PubMed] [Europe PMC] [Abstract]
  14. "Differential function of Tie2 at cell-cell contacts and cell-substratum contacts regulated by angiopoietin-1."Nat. Cell Biol. 10:513-526(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts."Nat. Cell Biol. 10:527-537(2008) [PubMed] [Europe PMC] [Abstract]
  16. "Tyrosine phosphatase beta regulates angiopoietin-Tie2 signaling in human endothelial cells."Angiogenesis 12:25-33(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Angiopoietin-1-induced ubiquitylation of Tie2 by c-Cbl is required for internalization and degradation."Biochem. J. 423:375-380(2009) [PubMed] [Europe PMC] [Abstract]
  18. "VE-PTP controls blood vessel development by balancing Tie-2 activity."J. Cell Biol. 185:657-671(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Angiopoietin 2 is a partial agonist/antagonist of Tie2 signaling in the endothelium."Mol. Cell. Biol. 29:2011-2022(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Tie2: a journey from normal angiogenesis to cancer and beyond."Histol. Histopathol. 23:773-780(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Tie2 is tied at the cell-cell contacts and to extracellular matrix by angiopoietin-1."Exp. Mol. Med. 41:133-139(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Control of vascular morphogenesis and homeostasis through the angiopoietin-Tie system."Nat. Rev. Mol. Cell Biol. 10:165-177(2009) [PubMed] [Europe PMC] [Abstract]
  24. "Angiopoietin-1/Tie2 receptor signaling in vascular quiescence and angiogenesis."Histol. Histopathol. 25:387-396(2010) [PubMed] [Europe PMC] [Abstract]
  25. "Targeting the ANGPT-TIE2 pathway in malignancy."Nat. Rev. Cancer 10:575-585(2010) [PubMed] [Europe PMC] [Abstract]
  26. "Structure of the Tie2 RTK domain: self-inhibition by the nucleotide binding loop, activation loop, and C-terminal tail."Structure 8:1105-1113(2000) [PubMed] [Europe PMC] [Abstract]
  27. "Crystal structures of the Tie2 receptor ectodomain and the angiopoietin-2-Tie2 complex."Nat. Struct. Mol. Biol. 13:524-532(2006) [PubMed] [Europe PMC] [Abstract]
  28. "Synthesis, structural analysis, and SAR studies of triazine derivatives as potent, selective Tie-2 inhibitors." Bioorg. Med. Chem. Lett. 17:2886-2889(2007) [PubMed] [Europe PMC] [Abstract]
  29. "Evolution of a highly selective and potent 2-(pyridin-2-yl)-1,3,5-triazine Tie-2 kinase inhibitor." J. Med. Chem. 50:611-626(2007) [PubMed] [Europe PMC] [Abstract]
  30. "Novel thienopyrimidine and thiazolopyrimidine kinase inhibitors with activity against Tie-2 in vitro and in vivo."Bioorg. Med. Chem. Lett. 19:6670-6674(2009) [PubMed] [Europe PMC] [Abstract]
  31. "Crystal structure of cytoplasmic kinase domain of Tie2 complexed with inhibitor CEP11207."Submitted (JAN-2010) to the PDB data bank
  32. "Vascular dysmorphogenesis caused by an activating mutation in the receptor tyrosine kinase TIE2."Cell 87:1181-1190(1996) [PubMed] [Europe PMC] [Abstract]
  33. "Allelic and locus heterogeneity in inherited venous malformations."Hum. Mol. Genet. 8:1279-1289(1999) [PubMed] [Europe PMC] [Abstract]
  34. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
  35. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
  36. "Hereditary cutaneomucosal venous malformations are caused by TIE2 mutations with widely variable hyper-phosphorylating effects."Eur. J. Hum. Genet. 18:414-420(2010) [PubMed] [Europe PMC] [Abstract]