Fibronectin (FN)

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FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein

Fibronectin (FN)

Fibronectin (Fn) is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matirx (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids.

The protein subunit is made up of three types of homologous structural repeats termed Fn type I, type II, and type III repeats. Multiple isoforms of the protein formed by alternative splicing at numerous sites, resulting in insertions of extra type III domains (EDA and ECB) or parts of the variable type III connecting segment (V/IIICS), have been identified. Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and many integrin receptors. It is involved in multiple cellular processes including cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA037Hu01 Recombinant Fibronectin (FN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA037Hu02 Recombinant Fibronectin (FN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA037Hu03 Recombinant Fibronectin (FN) Positive Control; Immunogen; SDS-PAGE; WB.
APA037Hu02 Active Fibronectin (FN) Cell culture; Activity Assays.
APA037Hu01 Active Fibronectin (FN) Cell culture; Activity Assays.
Antibodies PAA037Hu01 Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
MAA037Hu22 Monoclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
PAA037Hu02 Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
LAA037Hu71 Biotin-Linked Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC.
PAA037Hu03 Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
PAA037Hu04 Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
Assay Kits SEA037Hu ELISA Kit for Fibronectin (FN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA037Hu CLIA Kit for Fibronectin (FN) Chemiluminescent immunoassay for Antigen Detection.
HEA037Hu High Sensitive ELISA Kit for Fibronectin (FN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA037Mu01 Recombinant Fibronectin (FN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA037Mu02 Recombinant Fibronectin (FN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA037Mu01 Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
LAA037Mu71 Biotin-Linked Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC.
PAA037Mu02 Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
Assay Kits SEA037Mu ELISA Kit for Fibronectin (FN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA037Ra01 Recombinant Fibronectin (FN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA037Ra01 Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
LAA037Ra71 Biotin-Linked Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC.
LAA037Ra81 FITC-Linked Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IF.
MAA037Ra21 Monoclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
Assay Kits SEA037Ra ELISA Kit for Fibronectin (FN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Fibronectin (FN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Fibronectin (FN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Fibronectin (FN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Fibronectin (FN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Fibronectin (FN) ELISA Kit Customized Service Offer

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA037Po01 Recombinant Fibronectin (FN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA037Po01 Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
MAA037Po21 Monoclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
Assay Kits SEA037Po ELISA Kit for Fibronectin (FN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA037Bo01 Recombinant Fibronectin (FN) Positive Control; Immunogen; SDS-PAGE; WB.
NPA037Bo01 Native Fibronectin (FN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA037Bo01 Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
LAA037Bo71 Biotin-Linked Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC.
MAA037Bo21 Monoclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
Assay Kits SCA037Bo CLIA Kit for Fibronectin (FN) Chemiluminescent immunoassay for Antigen Detection.
SEA037Bo ELISA Kit for Fibronectin (FN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Chicken (Gallus)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA037Ga01 Recombinant Fibronectin (FN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA037Ga01 Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IP.
LAA037Ga71 Biotin-Linked Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC.
LAA037Ga81 FITC-Linked Polyclonal Antibody to Fibronectin (FN) WB; IHC; ICC; IF.
Assay Kits n/a CLIA Kit for Fibronectin (FN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Fibronectin (FN) ELISA Kit Customized Service Offer
  1. "Phenotypic and genetic alterations in mammary stroma: implications for tumour progression."Breast Cancer Res. 3:373-379(2001) [PubMed] [Europe PMC] [Abstract]
  2. "The expression of migration stimulating factor, a potent oncofetal cytokine, is uniquely controlled by 3'-untranslated region-dependent nuclear sequestration of its precursor messenger RNA."Cancer Res. 65:10742-10749(2005) [PubMed] [Europe PMC] [Abstract]
  3. "Vector-capping: a simple method for preparing a high-quality full-length cDNA library."DNA Res. 12:53-62(2005) [PubMed] [Europe PMC] [Abstract]
  4. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Human fibronectin is synthesized as a pre-propolypeptide."FEBS Lett. 207:145-148(1986) [PubMed] [Europe PMC] [Abstract]
  8. "Cloning and analysis of the promotor region of the human fibronectin gene."Proc. Natl. Acad. Sci. U.S.A. 84:1876-1880(1987) [PubMed] [Europe PMC] [Abstract]
  9. "Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene."EMBO J. 4:1755-1759(1985) [PubMed] [Europe PMC] [Abstract]
  10. "Primary structure of human plasma fibronectin. The 29,000-dalton NH2-terminal domain."J. Biol. Chem. 258:12670-12674(1983) [PubMed] [Europe PMC] [Abstract]
  11. "Mapping the collagen-binding site of human fibronectin by expression in Escherichia coli."EMBO J. 5:2825-2830(1986) [PubMed] [Europe PMC] [Abstract]
  12. "Primary structure of a DNA- and heparin-binding domain (Domain III) in human plasma fibronectin."J. Biol. Chem. 260:12136-12141(1985) [PubMed] [Europe PMC] [Abstract]
  13. "Identification of novel fibronectin fragments detected specifically in juvenile urine."FEBS J. 274:3939-3947(2007) [PubMed] [Europe PMC] [Abstract]
  14. "Human fibronectin: cell specific alternative mRNA splicing generates polypeptide chains differing in the number of internal repeats."Nucleic Acids Res. 12:5853-5868(1984) [PubMed] [Europe PMC] [Abstract]
  15. "Sequence analysis and in vivo expression show that alternative splicing of ED-B and ED-A regions of the human fibronectin gene are independent events."Nucleic Acids Res. 16:3545-3557(1988) [PubMed] [Europe PMC] [Abstract]
  16. "Transformed human cells produce a new fibronectin isoform by preferential alternative splicing of a previously unobserved exon."EMBO J. 6:2337-2342(1987) [PubMed] [Europe PMC] [Abstract]
  17. "Identification of a third region of cell-specific alternative splicing in human fibronectin mRNA."Proc. Natl. Acad. Sci. U.S.A. 84:7179-7182(1987) [PubMed] [Europe PMC] [Abstract]
  18. "The cell attachment domain of fibronectin. Determination of the primary structure."J. Biol. Chem. 257:9593-9597(1982) [PubMed] [Europe PMC] [Abstract]
  19. "Molecular cloning and nucleotide sequence of a cDNA clone coding for the cell attachment domain in human fibronectin."J. Biol. Chem. 258:10193-10196(1983) [PubMed] [Europe PMC] [Abstract]
  20. "Evolution of the fibronectin gene. Exon structure of cell attachment domain."J. Biol. Chem. 261:2113-2116(1986) [PubMed] [Europe PMC] [Abstract]
  21. "Primary structure of human plasma fibronectin. Characterization of a 38 kDa domain containing the C-terminal heparin-binding site (Hep III site) and a region of molecular heterogeneity."Biochem. J. 241:923-928(1987) [PubMed] [Europe PMC] [Abstract]
  22. "Human cellular fibronectin: comparison of the carboxyl-terminal portion with rat identifies primary structural domains separated by hypervariable regions."Biochemistry 24:2698-2704(1985) [PubMed] [Europe PMC] [Abstract]
  23. "Human plasma fibronectin. Demonstration of structural differences between the A- and B-chains in the III CS region."Biochem. J. 274:731-738(1991) [PubMed] [Europe PMC] [Abstract]
  24. "Human fibronectin: molecular cloning evidence for two mRNA species differing by an internal segment coding for a structural domain."EMBO J. 3:221-226(1984) [PubMed] [Europe PMC] [Abstract]
  25. "Human liver fibronectin complementary DNAs: identification of two different messenger RNAs possibly encoding the alpha and beta subunits of plasma fibronectin."Biochemistry 25:4936-4941(1986) [PubMed] [Europe PMC] [Abstract]
  26. "Novel cartilage-specific splice variants of fibronectin."Osteoarthritis Cartilage 10:528-534(2002) [PubMed] [Europe PMC] [Abstract]
  27. "Isolation and characterization of cDNA clones for human liver fibronectin."FEBS Lett. 186:31-34(1985) [PubMed] [Europe PMC] [Abstract]
  28. "Donor and acceptor splice signals within an exon of the human fibronectin gene: a new type of differential splicing."FEBS Lett. 207:287-291(1986) [PubMed] [Europe PMC] [Abstract]
  29. "Primary structure of human plasma fibronectin. Characterization of a 31,000-dalton fragment from the COOH-terminal region containing a free sulfhydryl group and a fibrin-binding site."J. Biol. Chem. 260:10320-10325(1985) [PubMed] [Europe PMC] [Abstract]
  30. "Isolation and characterization of cDNA clones for human and bovine fibronectins."Proc. Natl. Acad. Sci. U.S.A. 80:3218-3222(1983) [PubMed] [Europe PMC] [Abstract]
  31. "Tyrosine sulfation of proteins from the human hepatoma cell line HepG2."Proc. Natl. Acad. Sci. U.S.A. 82:7160-7164(1985) [PubMed] [Europe PMC] [Abstract]
  32. "Purification of a young age-related glycoprotein (Ugl-Y) from normal human urine."J. Biochem. 101:357-363(1987) [PubMed] [Europe PMC] [Abstract]
  33. "Fibulin binds to itself and to the carboxyl-terminal heparin-binding region of fibronectin."J. Biol. Chem. 267:20120-20125(1992) [PubMed] [Europe PMC] [Abstract]
  34. "Further characterization of the NH2-terminal fibrin-binding site on fibronectin."J. Biol. Chem. 269:31938-31945(1994) [PubMed] [Europe PMC] [Abstract]
  35. "Superfibronectin is a functionally distinct form of fibronectin."Nature 367:193-196(1994) [PubMed] [Europe PMC] [Abstract]
  36. "Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin."EMBO J. 17:1606-1613(1998) [PubMed] [Europe PMC] [Abstract]
  37. "A fibronectin fragment inhibits tumor growth, angiogenesis, and metastasis."Proc. Natl. Acad. Sci. U.S.A. 98:620-624(2001) [PubMed] [Europe PMC] [Abstract]
  38. "The type XIII collagen ectodomain is a 150-nm rod and capable of binding to fibronectin, nidogen-2, perlecan, and heparin."J. Biol. Chem. 277:23092-23099(2002) [PubMed] [Europe PMC] [Abstract]
  39. "Matrix-matrix interaction of cartilage oligomeric matrix protein and fibronectin."Matrix Biol. 21:461-470(2002) [PubMed] [Europe PMC] [Abstract]
  40. "In vitro localization of TIGR/MYOC in trabecular meshwork extracellular matrix and binding to fibronectin."Invest. Ophthalmol. Vis. Sci. 43:151-161(2002) [PubMed] [Europe PMC] [Abstract]
  41. "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."Proteomics 4:454-465(2004) [PubMed] [Europe PMC] [Abstract]
  42. "Anastellin, a fragment of the first type III repeat of fibronectin, inhibits extracellular signal-regulated kinase and causes G(1) arrest in human microvessel endothelial cells."Cancer Res. 65:148-156(2005) [PubMed] [Europe PMC] [Abstract]
  43. "Differential analysis of site-specific glycans on plasma and cellular fibronectins: application of a hydrophilic affinity method for glycopeptide enrichment."Glycobiology 15:1332-1340(2005) [PubMed] [Europe PMC] [Abstract]
  44. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
  45. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  46. "A novel role for fibronectin type I domain in the regulation of human hematopoietic cell adhesiveness through binding to follistatin domains of FLRG and follistatin."Exp. Cell Res. 312:434-442(2006) [PubMed] [Europe PMC] [Abstract]
  47. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  48. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  49. "A strategy for precise and large scale identification of core fucosylated glycoproteins."Mol. Cell. Proteomics 8:913-923(2009) [PubMed] [Europe PMC] [Abstract]
  50. "Regulation of p38 MAP kinase by anastellin is independent of anastellin's effect on matrix fibronectin."Matrix Biol. 28:101-109(2009) [PubMed] [Europe PMC] [Abstract]
  51. "Resveratrol-induced changes of the human adipocyte secretion profile."J. Proteome Res. 11:4733-4743(2012) [PubMed] [Europe PMC] [Abstract]
  52. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  53. "1H NMR assignment and secondary structure of the cell adhesion type III module of fibronectin."Biochemistry 31:2068-2073(1992) [PubMed] [Europe PMC] [Abstract]
  54. "The three-dimensional structure of the tenth type III module of fibronectin: an insight into RGD-mediated interactions."Cell 71:671-678(1992) [PubMed] [Europe PMC] [Abstract]
  55. "Solution structure of a pair of fibronectin type 1 modules with fibrin binding activity."J. Mol. Biol. 235:1302-1311(1994) [PubMed] [Europe PMC] [Abstract]
  56. "High-resolution structural studies of the factor XIIIa crosslinking site and the first type 1 module of fibronectin."Nat. Struct. Biol. 2:946-950(1995) [PubMed] [Europe PMC] [Abstract]
  57. "Solution structure of the glycosylated second type 2 module of fibronectin."J. Mol. Biol. 276:177-187(1998) [PubMed] [Europe PMC] [Abstract]
  58. "NMR structure of the human oncofoetal fibronectin ED-B domain, a specific marker for angiogenesis."Structure 7:381-390(1999) [PubMed] [Europe PMC] [Abstract]
  59. "Solution structure of a pair of modules from the gelatin-binding domain of fibronectin."Structure 7:1451-1460(1999) [PubMed] [Europe PMC] [Abstract]
  60. "The hairpin structure of the (6)F1(1)F2(2)F2 fragment from human fibronectin enhances gelatin binding."EMBO J. 20:1519-1529(2001) [PubMed] [Europe PMC] [Abstract]
  61. "NMR structure of human fibronectin EDA."J. Biomol. NMR 21:281-284(2001) [PubMed] [Europe PMC] [Abstract]
  62. "Anastellin, an FN3 fragment with fibronectin polymerization activity, resembles amyloid fibril precursors."J. Mol. Biol. 332:205-215(2003) [PubMed] [Europe PMC] [Abstract]
  63. "Pathogenic bacteria attach to human fibronectin through a tandem beta-zipper."Nature 423:177-181(2003) [PubMed] [Europe PMC] [Abstract]
  64. "Crystal structure of the tenth type III cell adhesion module of human fibronectin."J. Mol. Biol. 236:1079-1092(1994) [PubMed] [Europe PMC] [Abstract]
  65. "2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region."Cell 84:155-164(1996) [PubMed] [Europe PMC] [Abstract]
  66. "Crystal structure of a heparin- and integrin-binding segment of human fibronectin."EMBO J. 18:1468-1479(1999) [PubMed] [Europe PMC] [Abstract]
  67. "Structure and functional significance of mechanically unfolded fibronectin type III1 intermediates."Proc. Natl. Acad. Sci. U.S.A. 100:14784-14789(2003) [PubMed] [Europe PMC] [Abstract]
  68. "Interdomain association in fibronectin: insight into cryptic sites and fibrillogenesis."EMBO J. 26:2575-2583(2007) [PubMed] [Europe PMC] [Abstract]
  69. "The solution and crystal structures of a module pair from the Staphylococcus aureus-binding site of human fibronectin -- a tale with a twist."J. Mol. Biol. 368:833-844(2007) [PubMed] [Europe PMC] [Abstract]
  70. "Crystal structures of fibronectin-binding sites from Staphylococcus aureus FnBPA in complex with fibronectin domains."Proc. Natl. Acad. Sci. U.S.A. 105:12254-12258(2008) [PubMed] [Europe PMC] [Abstract]
  71. "Solution structure of the 11th FN1 domain from human fibronectin 1."Submitted (MAR-2008) to the PDB data bank
  72. "Identification and structural analysis of type I collagen sites in complex with fibronectin fragments."Proc. Natl. Acad. Sci. U.S.A. 106:4195-4200(2009) [PubMed] [Europe PMC] [Abstract]
  73. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
  74. "Mutations in FN1 cause glomerulopathy with fibronectin deposits."Proc. Natl. Acad. Sci. U.S.A. 105:2538-2543(2008) [PubMed] [Europe PMC] [Abstract]
  75. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]