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Angiogenin (ANG)

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RNASE5; Ribonuclease,RNase A Family 5

Angiogenin (ANG)
CCC A007
HGNC 483
MGI 88022
PubMed 23047679
RGD 1359373
UniProt P03950
Wiki Angiogenin

Angiogenin (Ang) is a small polypeptide that is implicated in angiogenesis (formation of new blood vessels) in tumor growth . However, angiogenin is unique among the many proteins that are involved in angiogenesis in that it is also an enzyme with an amino acid sequence 33% identical to that of bovine pancreatic ribonuclease (RNase) A).

Moreover, although Ang has the same general catalytic properties as RNase A - it cleaves preferentially on the 3' side of pyrimidines and follows a transphosphorylation/hydrolysis mechanism - its activity differs markedly both in magnitude and in specificity.Although angiogenin contains counterparts for the key catalytic residues of bovine pancreatic RNase A, it cleaves standard RNase substrates 105 - 106 times less efficiently than does RNase A.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA007Hu01 Recombinant Angiogenin (ANG) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA007Hu01 Polyclonal Antibody to Angiogenin (ANG) WB,IHC
MAA007Hu22 Monoclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
MAA007Hu21 Monoclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
MAA007Hu23 Monoclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
MAA007Hu24 Monoclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
Assay Kits SEA007Hu ELISA Kit for Angiogenin (ANG) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA007Mu01 Recombinant Angiogenin (ANG) Positive Control; Immunogen; SDS-PAGE; WB.
RPA007Mu02 Recombinant Angiogenin (ANG) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA007Mu01 Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
PAA007Mu02 Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
Assay Kits SEA007Mu ELISA Kit for Angiogenin (ANG) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA007Ra01 Recombinant Angiogenin (ANG) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA007Ra01 Polyclonal Antibody to Angiogenin (ANG) WB; IHC
Assay Kits n/a CLIA Kit for Angiogenin (ANG) CLIA Kit Customized Service Offer
n/a ELISA Kit for Angiogenin (ANG) ELISA Kit Customized Service Offer

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA007Po01 Recombinant Angiogenin (ANG) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA007Po01 Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
LAA007Po71 Biotin-Linked Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC.
MAA007Po21 Monoclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
Assay Kits SEA007Po ELISA Kit for Angiogenin (ANG) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA007Bo01 Recombinant Angiogenin (ANG) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA007Bo01 Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
LAA007Bo71 Biotin-Linked Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC.
LAA007Bo81 FITC-Linked Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IF.
MAA007Bo22 Monoclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
Assay Kits SEA007Bo ELISA Kit for Angiogenin (ANG) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Equus caballus; Equine (Horse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA007Eq01 Recombinant Angiogenin (ANG) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA007Eq01 Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Angiogenin (ANG) CLIA Kit Customized Service Offer
n/a ELISA Kit for Angiogenin (ANG) ELISA Kit Customized Service Offer
  1. "Sequence of the cDNA and gene for angiogenin, a human angiogenesis factor."Biochemistry 24:5494-5499(1985) [PubMed] [Europe PMC] [Abstract]
  2. "Diversifying selection of the tumor-growth promoter angiogenin in primate evolution."Mol. Biol. Evol. 19:438-445(2002) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Amino acid sequence of human tumor derived angiogenin."Biochemistry 24:5486-5494(1985) [PubMed] [Europe PMC] [Abstract]
  5. "Tissue distribution and developmental expression of the messenger RNA encoding angiogenin."Science 237:280-282(1987) [PubMed] [Europe PMC] [Abstract]
  6. "Angiogenin is a cytotoxic, tRNA-specific ribonuclease in the RNase A superfamily."J. Biol. Chem. 267:21982-21986(1992) [PubMed] [Europe PMC] [Abstract]
  7. "Identification of the nucleolar targeting signal of human angiogenin."Biochem. Biophys. Res. Commun. 203:1765-1772(1994) [PubMed] [Europe PMC] [Abstract]
  8. "Site-specific mutagenesis reveals differences in the structural bases for tight binding of RNase inhibitor to angiogenin and RNase A."Proc. Natl. Acad. Sci. U.S.A. 94:1761-1766(1997) [PubMed] [Europe PMC] [Abstract]
  9. "Superadditive and subadditive effects of 'hot spot' mutations within the interfaces of placental ribonuclease inhibitor with angiogenin and ribonuclease A."Biochemistry 38:9273-9285(1999) [PubMed] [Europe PMC] [Abstract]
  10. "The nuclear function of angiogenin in endothelial cells is related to rRNA production."Biochem. Biophys. Res. Commun. 294:287-292(2002) [PubMed] [Europe PMC] [Abstract]
  11. "Ribonuclease inhibitor regulates neovascularization by human angiogenin."Biochemistry 48:3804-3806(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Angiogenin-induced tRNA fragments inhibit translation initiation."Mol. Cell 43:613-623(2011) [PubMed] [Europe PMC] [Abstract]
  13. "Crystal structure of human angiogenin reveals the structural basis for its functional divergence from ribonuclease."Proc. Natl. Acad. Sci. U.S.A. 91:2915-2919(1994) [PubMed] [Europe PMC] [Abstract]
  14. "Molecular recognition of human angiogenin by placental ribonuclease inhibitor -- an X-ray crystallographic study at 2.0-A resolution."EMBO J. 16:5162-5177(1997) [PubMed] [Europe PMC] [Abstract]
  15. "Refined crystal structures of native human angiogenin and two active site variants: implications for the unique functional properties of an enzyme involved in neovascularisation during tumour growth."J. Mol. Biol. 285:1209-1233(1999) [PubMed] [Europe PMC] [Abstract]
  16. "Binding of phosphate and pyrophosphate ions at the active site of human angiogenin as revealed by X-ray crystallography."Protein Sci. 10:1669-1676(2001) [PubMed] [Europe PMC] [Abstract]
  17. "Crystallographic studies on the role of the C-terminal segment of human angiogenin in defining enzymatic potency."Biochemistry 41:2552-2562(2002) [PubMed] [Europe PMC] [Abstract]
  18. "The crystal structure of human angiogenin in complex with an antitumor neutralizing antibody."Structure 11:875-885(2003) [PubMed] [Europe PMC] [Abstract]
  19. "Crystallographic studies on structural features that determine the enzymatic specificity and potency of human angiogenin: Thr44, Thr80, and residues 38-41."Biochemistry 43:1230-1241(2004) [PubMed] [Europe PMC] [Abstract]
  20. "Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy -- characterization of histidine protonation states and pKa values."Eur. J. Biochem. 250:712-726(1997) [PubMed] [Europe PMC] [Abstract]
  21. "Mechanisms of loss of functions of human angiogenin variants implicated in amyotrophic lateral sclerosis."PLoS ONE 7:E32479-E32479(2012) [PubMed] [Europe PMC] [Abstract]
  22. "A novel candidate region for ALS on chromosome 14q11.2."Neurology 63:1936-1938(2004) [PubMed] [Europe PMC] [Abstract]
  23. "ANG mutations segregate with familial and 'sporadic' amyotrophic lateral sclerosis."Nat. Genet. 38:411-413(2006) [PubMed] [Europe PMC] [Abstract]
  24. "Angiogenin loss-of-function mutations in amyotrophic lateral sclerosis."Ann. Neurol. 62:609-617(2007) [PubMed] [Europe PMC] [Abstract]
  25. "Characterization of human angiogenin variants implicated in amyotrophic lateral sclerosis."Biochemistry 46:11810-11818(2007) [PubMed] [Europe PMC] [Abstract]
  26. "Identification of new ANG gene mutations in a large cohort of Italian patients with amyotrophic lateral sclerosis."Neurogenetics 9:33-40(2008) [PubMed] [Europe PMC] [Abstract]
  27. "A novel angiogenin gene mutation in a sporadic patient with amyotrophic lateral sclerosis from southern Italy."Neuromuscul. Disord. 18:68-70(2008) [PubMed] [Europe PMC] [Abstract]
  28. "SOD1, ANG, TARDBP and FUS mutations in amyotrophic lateral sclerosis: a United States clinical testing lab experience."Amyotroph. Lateral Scler. 13:217-222(2012) [PubMed] [Europe PMC] [Abstract]
  29. "Computational and functional characterization of Angiogenin mutations, and correlation with amyotrophic lateral sclerosis."PLoS ONE 9:E111963-E111963(2014) [PubMed] [Europe PMC] [Abstract]