PCR Mix System

Instruction manual

First Edition (Revised on April, 2016)

PRODUCT INFORMATION

PCR Mix System is 2 times of the concentration of PCR pre-mixed solution, which can be directly diluted to standard concentration. Just with adding template and primer, PCR reaction system can be quickly and easily completed, it will reduce pollution and operating errors in the experiment. The PCR product of 3' end contains A, it could be directly used for T/A cloning .

PRODUCT TYPE

Product nameSpecifications
2*PCR Mix System100 reactions
500 reactions
1000 reactions

STORAGE

Stored at -20°.

PRODUCT COMPONENTS

Taq DNA Polymerase0.1U/μl
dNTPs0.5mM
KCl100mM
MgSO43mM
Tris-HCl20mM

APPLICATION EXAMPLES

50ul PCR reaction system:

PCR Mix System (2x)25μl
Template10pg-1ug
Primer 10.1-1.0μM
Primer 20.1-1.0μM
ddH2OTo 50μl

Systems with different volume could also be prepared according to 50ul PCR reaction system shown above.