PCR Mix System
First Edition (Revised on April, 2016)
PCR Mix System is 2 times of the concentration of PCR pre-mixed solution, which can be directly diluted to standard concentration. Just with adding template and primer, PCR reaction system can be quickly and easily completed, it will reduce pollution and operating errors in the experiment. The PCR product of 3' end contains A, it could be directly used for T/A cloning .
|2*PCR Mix System||100 reactions|
Stored at -20°.
|Taq DNA Polymerase||0.1U/μl|
50ul PCR reaction system:
|PCR Mix System (2x)||25μl|
Systems with different volume could also be prepared according to 50ul PCR reaction system shown above.