Loading Control Antibodies
Instruction manual
First Edition (Revised on April, 2016)
PRODUCT INFORMATION
Immunogen: Recombinant protein
Immunoglobulin Type: IgG
Purification: Affinity Chromatography
Traits: Liquid
Concentration: 200µg/mL
UOM: 100µg
Applications: Loading Control of WB
RELEVANCE
Proteins expressed by specific housekeeping genes are commonly used as loading controls. Normally, these proteins are expressed at relatively constant levels in specific cells and tissues under most non-pathological conditions. Therefore, they are usually used as the reference to evaluate the consistency of loading samples and experimental operation in Western Blot.
APPLICATIONS
Western blotting: 0.5-2ug/ml
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
STORAGE
Store at 4oC for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.